英国圣安德鲁斯大学的Powis等3 还在人淋巴母细胞T细胞系Jurkat和CEM中,比较了莫能菌素和A23187的释放效果(图5)。
图5 通过纳米粒子跟踪分析(NTA)定量测定外泌体的释放。(c)将Jurkat细胞在含有50 μm NH4Cl,100 μm氯喹,4 μm莫能菌素或0.5 μm A23187(钙-镁混合盐)的无血清培养基中孵育过夜。NTA分析10000 g后的上清液。检测到的平均粒径为114 nm。(d)将Jurkat细胞与所示浓度的莫能菌素和A23187一起孵育过夜。NTA分析10000 g后的上清液。检测到的平均粒径为126 nm。 ◆参考文献1. Savina, A., Furlán, M., Vidal, M., & Colombo, M. I. (2003). Exosome release is regulated by a calcium-dependent mechanism in K562 cells. Journal of Biological Chemistry, 278(22), 20083-20090. 【被引次数553】2. Guo, B. B., Bellingham, S. A., & Hill, A. F. (2016). Stimulating the release of exosomes increases the intercellular transfer of prions. Journal of Biological Chemistry, 291(10), 5128-5137.【被引次数72】3. Soo, C. Y., Song, Y., Zheng, Y., Campbell, E. C., Riches, A. C., Gunn‐Moore, F., & Powis, S. J. (2012). Nanoparticle tracking analysis monitors microvesicle and exosome secretion from immune cells. Immunology, 136(2), 192-197. 【被引次数172】