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文献和实验, w is the width of the flow chamber, h is the height of the flow chamber, and μ is the viscosity of the perfusate (flow medium). 2) A typical value of viscosity (μ ) for medium used is 0.9 cP (0.009 g cm-1 s-1 ). Note
E.Z.N.A.® Total RNA Midi Kit Protocol for Eukaryotic Cells and Tissues
and add the entire mixture to the column. With the spin column inside the 15 ml collecting tube (supplied with kit), centrifuge at 4,000-5,000x g for 5 min at room temperature. Discard flow-through and proceed to step 4. 4. Wash column with RNA Wash
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
15. DPBS Without Calcium or Magnesium 16. TRIzol® LS Reagent 17. SuperScript® VILO™ cDNA Synthesis Kit 18. AccuPrime™ SuperMix I 19. Mouse Anti-Tra1-60 Antibody 20. Mouse Anti-Tra1-81 Antibody 21. Mouse Anti-SSEA
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