Gene Knockout Kit v2

A Novel Strategy for a Splice-Variant Selective Gene Ablation: The Example of the Versican V0/V2 Knockout

, we had to circumvent the early embryonic lethality of the complete knockout by employing a novel splice-variant-specific gene ablation approach. For this purpose, we introduced a preterm translational stop codon preceded by an ER-retention signal (KDEL

Gene Knockout Techniques(1) The Knockout Process

for mutations in unc-22Day 8 (Wednesday) unc-22 is a large gene that makes a good test target for mutagenesis. Mutations that knock out unc-22 produce a dominant "twitching" phenotype when the animals are exposed to 1% nicotine. The presence of twitchers

Gene Trap: Knockout on the Fast Lane

Gene trapping is a powerful tool to ablate gene function and to analyze in vivo promoter activity of the trapped gene in parallel. The gene trap strategy is not as commonly used as the conventional gene-targeting strategy, although it offers