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- 2025年11月18日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8°C
- 保质期：
1年
- 英文名：
Cell Counting Kit
- 库存：
500
- 供应商：
上海攸碧艾生物
- CAS号：
/
- 规格：
5ml/5*5ml/10*5ml
| 规格： | 5ml | 产品价格： | ￥190.0 |
|---|---|---|---|
| 规格： | 5*5ml | 产品价格： | ￥1248.0 |
| 规格： | 10*5ml | 产品价格： | ￥2144.0 |
CCK-8细胞增殖检测试剂说明书
操作方法：
细胞毒性检测
1. 接种细胞：接种对数期的细胞至96孔板，接种数量1~5×103个/孔，每孔100 μl，每组设置3~5个复孔，置于37℃，5% CO2培养箱；
2. 样品处理：待细胞贴壁后，加入不同浓度的样品处理细胞，然后置于培养箱中孵育一定的时间（如：24 h、48 h、72 h）；
3. CCK-8孵育：每孔加入10 μl的CCK-8试剂，轻轻混匀，并将培养板置于37℃，5% CO2培养箱中孵育1~4 h；（可进行预实验确定孵育时间）；
4. 上机检测：将孵育后的细胞培养板置于酶标仪上，在450 nm处检测其吸光值；
储存条件: 4℃保存，半年有效；-20℃保存，两年有效
2. 以Hela细胞为例，细胞按1.25×103 ~ 20×103个/孔的密度梯度接种至96孔板中，培养4 h后，待细胞完全贴壁，每孔加入10 μl的CCK-8试剂，轻轻混匀，置于培养箱中孵育1 h后，在450 nm处检测其吸光值，并制作标准曲线。
注意事项：
1. 使用前先将低温保存的试剂在室温条件下放置20-30 min。
2. 接种对数期生长的细胞，注意使细胞分散均匀，确保每个复孔中的细胞数量一致，减少误差。
3. 为了达到最佳的实验效果，建议先进行预实验，确定最佳的细胞接种密度及CCK-8试剂孵育时间；以贴壁细胞为例，接种密度约1~5×103个/孔；孵育时间为1~4 h，多数细胞孵育约30 min即可观察到明显的颜色变化。
4. 设置空白对照，在无细胞的培养基中加入CCK-8检测，去除本底对吸光值的干扰。
5. 如果OD值太高，建议降低细胞接种密度或减少CCK-8试剂孵育时间。
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Overview Count the number of viable cells in a culture via dilution plating. The following assumes E. coli cells but it should apply to any type of cells. Materials LB agar plate LB media for dilution
Cell counting with an hemacytometer.
% variability or more) Hycor Biomediacal Inc. Garden grove CA 92841 Hemacytometer Fisher scientific No 02671 5 Dilute your cell sample in Trypan Blue dye exclusion medium. Dead cells will be blue, live cells will be birefringent. Suggested dilution
Cell counting with an hemacytometer.
Inc. Garden grove CA 92841 Hemacytometer Fisher scientific No 02671 5 Dilute your cell sample in Trypan Blue dye exclusion medium. Dead cells will be blue, live cells will be birefringent. Suggested dilution 1:20 (5µl cell suspension in 95 µl Trypan Blue
