- ￥148.80
- 攸碧艾/YOBIBIO
- China
- U31-301C
- 2026年02月10日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°c
- 保质期：
1年
- 英文名：
/
- 库存：
500
- 供应商：
上海攸碧艾生物
- CAS号：
/
- 规格：
100ml
产品描述： 本产品由10kU/ml青霉素和10mg/ml链霉素溶于0.85%氯化钠溶液配制而成。青霉素 (Penicillin) 通过抑制细菌细胞壁肽聚糖的合成导致细菌破裂死亡，对革兰氏阳性菌具有较强的抗菌作用。链霉素 (Streptomycin) 通过与细菌核糖体的30S亚单位结合，干扰细菌蛋白质合成，抑制细菌生长繁殖，对革兰氏阳性菌和部分耐药菌株具有较好的抑制效果。两者联合使用预防细胞培养受到细菌污染。
在细胞培养中推荐的青霉素的工作浓度为100U/ml，链霉素的工作浓度为0.1mg/ml，即本产品按照100倍稀释使用即可。100ml本产品可以配制10L细胞培养液。
本产品是专门用于细胞培养的双抗，经过0.1μm过滤除菌，可以直接添加到细胞培养液中使用。
注意事项：
1、本产品4℃解冻后混匀使用，建议分装冻存于-20℃，避免反复冻融。
2、在使用本产品过程中要注意无菌操作，避免被微生物污染。
3、抗生素对于某些细胞系（比如干细胞或原代细胞等）可能具有一定的毒性，建议先进行一定的培养测试。
4、本产品仅作体外科研使用，不可用于临床诊断或治疗。
5、实验员请穿实验服并佩戴一次性手套操作。
为确保信息准确，建议您以随产品附带的纸质说明书为准。电子文档内容可能因版本更新而存在延迟，仅供参考。
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文献和实验【1】In Situ Piezoelectric-Catalytic Anti-Inflammation Promotes the Rehabilitation of Acute Spinal Cord Injury in Synergy
Advanced Materials ( IF 26.8 ) Pub Date : 2024-02-01 , DOI: 10.1002/adma.202311429
Yanling You 1, 2 , Junjie Jiang 3 , Gang Zheng 4 , Zhixin Chen 1, 2 , Ya-Xuan Zhu 5 , Hongshi Ma 1 , Han Lin 1, 2, 5 , Xiang Guo 4 , Jianlin Shi 1, 2, 5
【2】2D Black Ferroelectric Perovskite Nanocatalysts Enable Defect Modulation‐Augmented Piezocatalytic Glioma Therapy
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Huan Li 1 , Chao Ma 2 , Linqi Wang 2 , Tong Li 2 , Peng Li 2 , Yu Hu 2 , Xinyue Dai 3 , Dejun Wu 2 , Meiqi Chang 4 , Yu Chen 3 , Tao Xu 5
【3】Construction of a two-dimensional artificial antioxidase for nanocatalytic rheumatoid arthritis treatment
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Bowen Yang 1, 2 , Heliang Yao 1 , Jiacai Yang 1, 2 , Chang Chen 1, 2 , Jianlin Shi 1, 3
【4】Maternal Lactobacillus amylovorus-derived extracellular vesicles modulate M2 macrophage polarization to promote early-life gut development in offspring
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【5】Photothermal H2S generation promotes the function restoration of spinal cord from acute injury
Nano Today ( IF 10.9 ) Pub Date : 2024-12-31 , DOI: 10.1016/j.nantod.2024.102625
Yihan Chen , Yuanqing Ding , Min Ge , Ya-Xuan Zhu , Yanling You , Zhixin Chen , Yiming Tao , Rong Xie , Han Lin , Jianlin Shi
【6】Palmitoyltransferase ZDHHC6 promotes colon tumorigenesis by targeting PPARγ-driven lipid biosynthesis via regulating lipidome metabolic reprogramming
Journal of Experimental & Clinical Cancer Research ( IF 12.8 ) Pub Date : 2024-08-16 , DOI: 10.1186/s13046-024-03154-0
Junqi Shan 1 , Xinyu Li 2 , Runqi Sun 3 , Yao Yao 1 , Yan Sun 4 , Qin Kuang 4 , Xianling Dai 4 , Yanlai Sun 1
【7】Antioxidative Aortic Aneurysm Therapy by a Mn–N4 Biomimetic Site-Engineered Nanocatalyst
ACS Nano ( IF 16 ) Pub Date : 2025-02-24 , DOI: 10.1021/acsnano.4c15805
Di Jiang 1, 2, 3, 4 , Bowen Yang 1, 2 , Jianlin Shi 1, 2
【8】Ultrasound‐Assisted H2 Transmitter Enables Hydrogen‐Gene Therapy to Prevent Anesthesia/Surgery‐Induced Cognitive Impairment
Advanced Science ( IF 14.1 ) Pub Date : 2025-03-11 , DOI: 10.1002/advs.202414397
Ruonan Zhan 1 , Yan Fang 2 , Chuyun Lou 3 , Nan Chen 1 , Xuan Mo 1 , Bo Jiao 1 , Mengke Liu 1 , Yangxi Zhao 1 , Weichen Xu 4 , Huixiong Xu 5 , Haohao Yin 5, 6 , Yi Zhang 1
【9】Mild magnetic hyperthermia-activated immuno-responses for primary bladder cancer therapy
Biomaterials ( IF 12.9 ) Pub Date : 2024-02-21 , DOI: 10.1016/j.biomaterials.2024.122514
Fenggang Qi 1 , Qunqun Bao 2 , Ping Hu 3 , Yuedong Guo 2 , Yang Yan 4 , Xudong Yao 4 , Jianlin Shi 5
【10】Water-Enabled H2 Generation from Hydrogenated Silicon Nanosheets for Efficient Anti-Inflammation
Journal of the American Chemical Society ( IF 15.6 ) Pub Date : 2022-07-13 , DOI: 10.1021/jacs.2c04412
Yanling You 1, 2 , Ya-Xuan Zhu 3 , Junjie Jiang 4 , Min Wang 1 , Zhixin Chen 1, 2 , Chenyao Wu 1 , Jie Wang 1 , Wujie Qiu 1 , Deliang Xu 1 , Han Lin 1, 3 , Jianlin Shi 1, 2, 3
【11】Nanocatalytic antioxidation synergizes cardioprotection and antifibrosis in cardiac injury
Nano Today ( IF 10.9 ) Pub Date : 2025-06-02 , DOI: 10.1016/j.nantod.2025.102820
Ruixuan He , Bowen Yang , Jianlin Shi
【12】Novel monomers recipe derived from Shengji-Huayu formula targeting PI3K/Akt signaling pathway for diabetic wound healing based on accurate network pharmacology
Journal of Ethnopharmacology ( IF 5.4 ) Pub Date : 2025-02-17 , DOI: 10.1016/j.jep.2025.119509
Sheng Hu 1 , Fang Shen 2 , Ning Jia 1 , Caiyun Zhang 1 , Xinxin Wu 1 , Wencheng Jiang 2 , Bin Li 1 , Qilong Chen 1
【13】Fermented Cordyceps Powder alleviates silica-induced inflammation and fibrosis by inhibiting M1 macrophage polarization via the HMGB1-TLR4-NF-κB pathway
Journal of Ethnopharmacology ( IF 5.4 ) Pub Date : 2025-03-14 , DOI: 10.1016/j.jep.2025.119631
Shuangshuang Pu 1 , Xiangjing Meng 2 , Yushan Shi 1 , Ning Huang 1 , Chunlai Zhang 1 , Aimei Pang 1 , Hua Shao 2 , Qiang Jia 2
【14】Isocitrate dehydrogenases 2-mediated dysfunctional metabolic reprogramming promotes intestinal cancer progression via regulating HIF-1A signaling pathway
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Shixiong Liu 1 , Yun Zhou 2 , Yarong Chen 3 , Yuqin Qiao 3 , Lumucao Bai 3 , Shenhua Zhang 4 , Dongfang Men 4 , Haibu Zhang 4 , Fen Pan 4 , Yongshen Gao 4 , Jijing Wang 4 , Yuping Wang 5
【15】Suppression of JAK2/STAT3 Pathway by Notoginsenoside R1 Reduces Epithelial-Mesenchymal Transition in Non-small Cell Lung Cancer
Molecular Biotechnology ( IF 2.5 ) Pub Date : 2024-04-02 , DOI: 10.1007/s12033-024-01136-3
Min Wan 1 , Hong Yu 1 , Haoqing Zhai 2
【16】The Role of Emodin in the Treatment of Bladder Cancer based on Network Pharmacology and Experimental Verification
Combinatorial Chemistry & High Throughput Screening ( IF 1.7 ) Pub Date : 2024-03-19 , DOI: 10.2174/0113862073294990240122140121
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【18】Fermented Cordyceps Powder alleviates silica-induced inflammation and fibrosis by inhibiting M1 macrophage polarization via the HMGB1-TLR4-NF-κB pathway
Journal of Ethnopharmacology ( IF 5.4 ) Pub Date : 2025-03-14 , DOI: 10.1016/j.jep.2025.119631
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【20】EGCG attenuates BPA -induced male reproductive toxicity by regulating the blood-testis barrier by suppressing autophagy via AMPK/AKT/mTOR signaling pathway.
Comparative Biochemistry and Physiology C: Toxicology & Pharmacology ( IF 4.3 ) Pub Date : 2025-07-22 , DOI: 10.1016/j.cbpc.2025.110298
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Journal of Molecular Histology ( IF 2.2 ) Pub Date : 2025-06-27 , DOI: 10.1007/s10735-025-10453-z
The enzyme penicillin G acylase (PGA) is currently employed at an industrial scale in the hydrolysis of penicillin and cephalosporin G. Here, we describe the preparation of a new immobilized preparation of the enzyme that yields derivatives
A Method to Assay Penicillin-Binding Proteins
Key enzymes that assemble the bacterial cell wall are also the target of the Β-lactam class of antibiotics. The covalent binding of labeled penicillin to these proteins has been used in numerous studies in drug discovery, antibiotic
用加有青霉素的培养基来选择细菌的营养缺陷型的一种方法。也就是由于青霉素是细菌细胞壁的合成抑制剂，利用其可杀死正在繁殖的细菌的性质，对所有青霉素敏感菌的一种方便的选择法。例如将细菌先在完全培养基中培养24小时，离心分离菌体，即用生理盐水（ 0.85％— 0.95％ NaCl溶液）反复离心洗涤 2— 3次，然后在加有青霉素（ 100— 300单位 /立升）的基本培养基中于最适温度中培养 24小时。这时不是营养缺陷型的原来野生型细胞会因不断分裂而被青霉素所杀死。然后将菌悬液放在完全琼脂
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