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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
25 mM Tris pH=8.0 1M 1,25 ml MPS2 for 10 ml 0,2 N NaOH 10N 200 ml 1% SDS 10% 1 ml H2O 8,8 ml MPS3 for 100 ml 5 M potassium acetate 60 ml glacial acetic acid 11,5 ml H2O 28,5ml
Characterization of Glycoprotein Biopharmaceutical Products by Caliper LC90 CE-SDS Gel Technology
Over the last decade, science has greatly improved in the area of protein sizing and characterization. Efficient high-throughput methods are now available to substitute for the traditional labor-intensive SDS-PAGE methods, which alternatively
or glass dish by aspirating off solutions rather than transferring the gel. SDS gel electrophoresis Prepare and run the gel using the standard sds-page procedure. Use 120 ng of protein standard (consisting of 6 to 8 proteins
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