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文献和实验the AllPrep DNA/RNA 96 Kit. Six targets (195-1150 bp) were amplified by multiplex PCR and then analyzed on the QIAxcel system. Li: liver; Ki: kidney; Lu: lung; In: intestine; Sp: spleen; NTC: no template control (shows upper and lower markers). Total
Clean-up Spin Protocol For DNA fragments (30bp-100 bp) 1) Add 3 x volume of buffer P3, 3 x volume of isopropanol to the enzymatic reaction and mix throughly with pipetting or vortex. The maximum volume of the reaction can be processed
for 5 min. Transfer 600 μL the supernatant into a new 2 ml tube and add 200 μL Buffer SP2. Mix the sample throughly by vortexing. 5. Incubate on ice for 5 minutes. Centrifuge at 13,000 x g in a microcentrifuge for 5 minutes. 6. Carefully
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