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文献和实验Gene Editing in One‐Cell Embryos by Zinc‐Finger and TAL Nucleases
) H 2 O, embryo‐tested (Sigma, cat. no. W1503) Disodium EDTA (Sigma, cat. no. E4884)
Sample Preparation for Microinjection
C (10,000 x g). Chill supernatant or load directly into capillary. DNA [5] Purification The highest expression level of microinjected plasmid DNA is achieved when the DNA has been purified by CsCl ethidium bromide gradient
实验原理 Add a mixture of biotinylated monoclonal antibodies against non-DC cells to your starting sample. Add Depletion MyOne™ SA Dynabeads® and allow them to bind to the non-DCs during a short incubation. Separate
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