相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
99
- 英文名:
dTTP-ONH2
- CAS号:
TONH2-101
- 保质期:
1-2年
- 供应商:
北京孚博
- 保存条件:
4°/-20等
- 规格:
5 µmoles
美国Firebird Biomolecular Sciences 为科研、合成生物学、临床诊断等研究机构单位提供创新试剂。
Firebird delivers reagent innovations to the research, synthetic biology, and clinical diagnostics communities.
Nucleic acid analogs for:
DNA sequencing
Diagnostics
SNP detection
Synthetic Biology
我们优势进口Firebird Biomolecular Sciences的产品,价格优惠,货期2-4周,质量和售后有保障!
孚博生物提供合成可逆终止剂 dNTP-ONH2的订购和定制服务,欢迎联系我们了解更多信息!
厂家有4种类型的合成可逆终止剂 dNTP-ONH2:
The ONH2 170 series: Ready to use, untagged
The ONH2 100 series: Oxime blocked, untagged
The ONH2 DT series: Diol linked tags
The ONH2 DS series: Disulfide linked tags
Reversible terminators: Oxime blocked, untagged
Reversible terminators with a free 3'-ONH2 are ready to use and do not require deprotection or further treatment before use.
Thymine,货号 TONH2-101,5 µmoles $900
Cytosine,货号 CONH2-102,5 µmoles $1050
Adenine,货号 AONH2-103,5 µmoles $1050
Guanine,货号 GONH2-104,5 µmoles $1050
Reversible terminators: Oxime blocked, untagged
Reversible terminators are also available with the 3'-ONH2 protected as the acetoxime, which are more suitable for long term storage than our ready to use reversible terminators. For enzymatic incorporation, the oxime must be deprotected to the free 3' ONH 2 before use, which can be achieved in situ with buffered aqueous methoxylamine.
Thymine,货号:TONH2-101,5 µmoles $900
Cytosine,货号:CONH2-102,5 µmoles $1050
Adenine,货号:AONH2-103,5 µmoles $1050
Guanine,货号:GONH2-104,5 µmoles $1050
Reversible terminators: diol linked tags
Firebird offers reversible terminators with a 3'-ONH2 (protected as the acetoxime ) and a diol linker carrying a free amino group, to which a tag (fluorescent dye or other moiety) can be attached. The diol can be rapidly cleaved with dilute aqueous periodate.
Thymine,货号 TONH2-DT,1 µmole $1750
Cytosine,货号 CONH2-DT,1 µmole $1900
Adenine,货号 AONH2-DT,1 µmole $2200
Guanine,货号 GONH2-DT,1 µmole $2300
Reversible terminators: disulfide-linked tags
Firebird offers reversible terminators with a 3'-ONH2 (protected as the acetoxime) and a disulfide linker carrying a free amino group, to which a tag (fluorescent dye or other moiety) can be attached. The disulfide can be rapidly cleaved with phosphine or thiol reagents.
Thymine,货号 TONH2-ST,1 μmole $ 1750
Cytosine,货号 CONH2-ST,1 μmole $ 2000
Adenine,货号 AONH2-ST,1 μmole $ 2400
Guanine,货号 GONH2-ST,1 μmole $ 2500
For enzymatic incorporation, the oxime must be deprotected to the free 3'-ONH2 before use, which
can be achieved in situ with buffered aqueous methoxylamine.
孚博生物常备库存产品
品牌、货号、名称、包装如下:
Toxin technology#BT202,SEB,1mg;
Toxin technology#BT202red,SEB(Staphylococcal Enterotoxins B),1mg;
Toxin technology#ET404,SEE,100ug;
Toxin technology#DT303,SED,100ug;
Toxin technology#TT606,TSST-1,100ug;
Toxin technology#AT101,SEA,100ug;
Toxin technology#HT101,Alpha Hemolysin highly purified,100ug;
Toxin technology#HP101,Alpha Hemolysin - partially purified,10000Units;
IRA#SE-121-0.72mg,17β-ESTRADIOL,25片;
Listbio#150,Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae,1mg;
visual protein#IF01-4N,ImmunoFas Adjuvant,1套;
visual protein#HB01-1L,杂交瘤促进生长因子,50mg;
visual protein#BF20-50P,无蛋白封闭缓冲液,20片;
Research Diets#D12492,60%高脂饲料(research diets),12.5KG
Hooke Laboratories:
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验firebird
序列自动测定仪问世。化学降解法是在DNA的片段的5`端标记核素,然后用专一性化学试剂将DNA特异地降解,在电泳和自显影后,可得到从标记端延伸的片段供测读序列和进行比较。一般能读出200-250个核苷酸序列。双脱氧法是采用核苷酸链终止剂,如:2`,3`-双脱氧核苷三磷酸ddNTP(如ddTTP、ddTTP、ddGTP和ddCTP中的一种)掺入到DNA链中以终止链的延长,与掺入4种正常的dNTP的混合物分成四组进行反应,这样可得到一组结尾长衙不一、不同专一性核苷酸链终止剂结尾的DNA片段,经凝胶
(Sanger等,1977 ),酶法DNA序列测定技术才得到广泛应用。2",3"ddNTP与普通dNTP不同之处在同它们在脱氧核糖的3" 位置缺少一个羟基。它们可以在DNA聚合酶作用下通过其5" 三磷酸基团掺入到正在增长的DNA链中,但由于没有3"羟基,它们不能同后续的dNTP形成磷酸二酯链,因此,正在增长的DNA链不可能继续延伸。这样,在DNA合成反应混合物的4种普通dNTP中加入少量的一种ddNTP后, 链延伸将与偶然发生但却十分特异的链终止展开竞争,反应产物是一系列的核苷酸链,其长度取决于从用以起始
因组研究等。 Solexa:可逆终止化学反应 DNA 片段加上接头之后,可以随机的附着于玻璃表面(Flow cell),并且在固相的表面经过桥式扩增。这样就形成了数千份相同的单分子簇,被用做测序模板。测序采取边合成边测序的方法,和模板配对的 ddNTP 原料被添加上去,不配对的 ddNTP 原料被洗去,成像系统能够捕捉荧光标记的核苷酸。随着 DNA 3'端的阻断剂的去除,下一轮的延伸就可以进行。 和焦磷酸测序不同,每次 DNA 只能延伸一个核苷酸。Solexa 的读长在 100






![Cyclo[2-methylalanyl-L-phenylalanyl-D-prolyl-(aS,2S)-a-amino-h-oxo-2-oxiraneoctanoyl]](https://img1.dxycdn.com/p/s14/2025/0214/538/8670428594612820981.jpg!wh200)




