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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 英文名:
1M Tris-HCl,pH8.8
- 保质期:
1年
- 供应商:
上海信裕生物科技有限公司
- 保存条件:
常温
- 规格:
100mL;500mL
产品简介
用于配制SDS-PAGE或native PAGE的下层胶,进口试剂配制。
Tris也称Tris base或2-Amino-2-(hydroxymethyl)-1,3-propanediol。分子式为C4H11NO3,分子量121.14,纯度>99.9%。
注意事项
1)对人体有刺激性,请注意适当防护。
2)为了您的安全和健康,请穿实验服并戴一次性手套操作。
Tris, or tris(hydroxymethyl)aminomethane, or known during medical use as tromethamine or THAM, is an organic compound with the formula (HOCH2)3CNH2. It is extensively used in biochemistry and molecular biology as a component of buffer solutions such as in TAE and TBE buffers, especially for solutions of nucleic acids.
"Tris" is a chemical compound used as a buffer. The full name is tris(hydroxymethyl)aminomethane. Tris has the ability to absorb counter ions (+H and -OH) so as to help keep the solution that they are in at a stable pH level. When the pH of Tris is set using HCl (hydrochloric acid) the buffer is called Tris HCl.
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文献和实验Clonality - X Chromosome Inactivation Assay
can utilize X chromosome inactivation (methylation) to determine the clonality status of a tumor or premalignant lesion in females. The technique is based on a methylation-sensitive restriction enzyme and analysis of a polymorphic locus on the X chromosome
冷却至室温后再调定pH值,因为Tris溶液的pH值随温度的变化差异很大,温度每升高1℃,溶液的pH值大约降低0.03个单位。 2)10×TE Buffer (pH7.4, 7.6, 8.0) 组份浓度:100 mM Tris-HCl, 10 mM EDTA 配制量:1 L 配制方法: 1. 量取下列溶液,置于1 L烧杯中。 1M Tris-HCl Buffer(PH7.4,7.6,8.0) 100ml 500mM EDTA(PH8.0) 20ml 2. 向烧杯中加入约
Glucose 2.25 g Store at 4 C. 50 mM Tris pH 8.0 10 mM EDTA 50 mM 1 M Tris pH 8.0 5 ml 1 mM 0.5 M EDTA 2 ml H2O up to 100 ml Store at 4 C. 1 M Tris pH 8.0, 1.5 M NaCl 1X 1L 1X 2L 1X 1M Tris-HCl 88.8 g Tris-HCl 178 g 53.0 g
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