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- 文献和实验
- 技术资料
- 保存条件:
4℃
- 保质期:
1-2 years
- 英文名:
DotDiver HepAK 10
- 库存:
999
- 供应商:
Eagle Biosciences Inc.
- 规格:
96T or More
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文献和实验) 1-8μl Calf Thymus DNA (0.5ug/ul) 2μl TE buffer 0-7μl Total Volume 10μl Add 1ul of 4% Formic Acid and incubate for 25 min at 37℃. During this incubation, add 15 μl of stock piperidine to 135 μl
Ref. Seed and Sheen, Gene 67, 271-277 (1988) Normal reaction is Chloramphenicol + Acetyl-CoA -> Acetylchloramphenicol Assay uses butyryl-CoA (more hydrophobic) 14C-Cm+ Bu-CoA->Bu-14C-Cm Km (Cm) 10 µM Km (Ac-CoA) 50µM The 14C- Butyrylated Cm
Blunt PCR克隆乃利用传统T4连接酶方法,但载体和Topo平端的一样,含ccdB基因,所以同样可降低菌落背景,易于筛选。 表10 PCR克隆试剂盒的选择 Amplification Enzyme Application of Cloned PCR Product Ligation Method Product
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