PspEI内切酶北京品牌

特别提示：包括PspEI内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：PspEI内切酶
英文名称：PspEI
产品货号：GS2140
产品规格：1kU

储存条件：冷冻（-20℃）

使用说明

High enzyme concentration may result in star activity.

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sites	G↑GTNACC
来源	Pseudomonas species E
Assayed on	Lambda DNA
Optimal buffer	B?(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;1 mM DTT.)
Optimal temperature	37℃
Storage conditions	10mMTris-HCl(pH 7.5);50 mM KCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol;Store at -20℃.
Ligations	After 5-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 10 u.a.of enzyme for 16hoursat 37℃.
Methlation effect	Methylation sensitivity not tested
Thermal inactivation	enzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible Ends	BstEII?,BstPI,Eco065I,Eco91I,EcoO65I

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