- ¥550
- 信裕生物/XY-Bioscience
- XY-6207
- 进口/国产
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海信裕生物科技有限公司
- 库存：
50
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
一年
- 抗体英文名：
ANO1/Anoctamin 1 Rabbit Polyclonal Antibody
- 抗体名：
ANO1/Anoctamin 1 Rabbit Polyclonal Antibody
- 保存条件：
ANO1/Anoctamin 1 Rabbit Polyclonal Antibody -20ºC保存，Western一抗稀释液-20ºC或4ºC保存，一年有效。Western一抗稀释液优先推荐4ºC保存，长期不使用可以考虑-20ºC保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:1000-1:2000 | - | 1:100-1:200 | - | - | - | - | - |
抗体详细信息如下:
| About this Antibody | |
| Name | ANO1/Anoctamin 1 Rabbit Polyclonal Antibody |
| Category | Rabbit Polyclonal Antibody (pAb); Primary antibody |
| Isotype | IgG |
| Purification method | Affinity purification |
| Positive samples | LO2, HeLa, BT-474, Mouse lung, Mouse eye, Mouse kidney, Rat liver, Rat skeletal muscle |
| Cellular location | Cell membrane, Cytoplasm, Multi-pass membrane protein |
| Customer validation | - |
| About the Immunogen | |
| Immunogen | Recombinant fusion protein of human ANO1 (NP_060513.5). |
| Sequence | FKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKLIRYLKLKQQSPPDHEECVKRKQRYEVDYNLEPFAGLTPEYME |
| Gene ID | 55107 |
| Swiss Prot | Q5XXA6 |
| Synonyms | ANO1; DOG1; ORAOV2; TAOS2; TMEM16A; anoctamin-1 |
| Category | Transmembrane Transporters; Neuronal Signaling |
| Background | ANO1/Anoctamin 1, also called TMEM16A for Transmembrane Member 16A, is a plasma membrane calcium-activated chloride channel. It is essential for chloride secretion from epithelial tissues, and has been shown to be a specific marker for cells of Cajal, the pacemaker cells that control smooth muscle contraction. Anoctamin 1 knockout mice exhibit an altered gastric smooth muscle rhythmic contraction. More recently, research studies have identified Anoctamin 1 as a heat sensor in nociceptive neurons. Heat above 44ºC triggers anoctamin 1-dependent depolarization, contributing to the mediation of thermal nociception. |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| XY-6207 | ANO1/Anoctamin 1 Rabbit Polyclonal Antibody | 50μl |
| XY-050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
保存条件：
ANO1/Anoctamin 1 Rabbit Polyclonal Antibody -20ºC保存，Western一抗稀释液-20ºC或4ºC保存，一年有效。Western一抗稀释液优先推荐4ºC保存，长期不使用可以考虑-20ºC保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
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