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- xy-34R
- 2025年07月15日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液态/粉状
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
- Neurofilament H Phosphorylated (SMI 34) Monoclonal Antibody保存于运输说明：
- Store at -20°C
- 详细信息：
-
Description 
Monoclonal Antibody to Phosphorylated Neurofilaments H and M Clone SMI-34 Form Ascites Fluid (contains 0.01M sodium azide) Host Mouse Species Reactivity Mammalian IsoType Neurofilament H Phosphorylated (SMI 34) Monoclonal AntibodyIgG1 Specificity SMI 34 reacts with a phosphorylated epitope in extensively phosphorylated neurofilament H and, to a lesser extent, with neurofilament M in most mammalian species, as well as in chicken. Immunocytochemically, SMI 34 reacts broadly with thick and thin axons and some dendrites such as basket cell dendrites, but not Purkinje cell dendrites. Nerve cell bodies are generally unreactive. Other cells and tissues are unreactive except for peripheral axons. Phosphatase treatment of tissue sections or Western blots abolishes reaction with SMI 34. Staining is unaffected by trypsin. In pathological conditions, reaction with SMI 34 may be found also in neuronal cell bodies. In Alzheimers disease, SMI 34 reacts with intraneuronal tangles but does not react with extraneuronal (ghost) tangles. It is the only antiphosphoneurofilament that reacts regularly with Alzheimer tangles. It does not cross-react at concentrations of 1:20,000 with Alzheimer MAP-Tau in immunoblots, demonstrating, thereby, the presence of neurofilaments in Alzheimer tangles. Uses This antibody is effective in immunoblotting, immunocytochemistry and ELISA. These antibodies can be used for neurofilament detection in trypsin-treated tissue. Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. - Western blot: 1:1,000
- Immunohistochemistry: 1:1,000
- ELISA: 1:1,000
Neurofilament H Phosphorylated (SMI 34) Monoclonal AntibodyThe extent of permissible dilution of SMI 34 beyond those recommended for general application depends upon nature and concentration of the antigen examined, species of the antigen, method of fixation and kind of section examined.
Tissue Preparation: SMI 34 reacts on Western blots, paraffin, vibratome and frozen tissue sections as well as with neuronal cell cultures. Tissues and cultures can be fixed in a variety of paraformaldehyde or formaldehyde-containing fixatives including Bouins. "Recovery" of antigen in formalin-fixed tissue can be accomplished by autoclaving of deparaffinized sections in distilled water (Shin et al, Lab Invest, 64:693, 1991) or by boiling sections or tissue blocks immersed in tris buffered saline, pH 9.0, in a microwave oven for 15 min (Evers and Uylings, J Neurosci Methods 72:197, 1997). Post-fixation in cold methanol or methanol/hydrogen peroxide permits access of SMI 34 in frozen sections or thick sections of tissue and in tissue cultures.Storage Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. References Giasson BI, Mushynski WE: Aberrant stress-induced phosphorylation of perikaryal neurofilaments. J Biol Chem 271:30404, 1996.
Vickers JC, Morrison JH: Aberrant neurofilament protein immunoreactivity in hippocampal pyramidal neurons in Alzheimers disease. J Neuropath Exper Neurol 51:319, 1992.
Zhang H, Sternberger NH, Rubinstein LJ, Herman MM, Binder LI, Sternberger LA: Abnormal processing of multiple proteins in Alzheimer disease. Proc Natl Acad Sci USA 86:8045-8049, 1989.
Cork LC, Sternberger NH, Sternberger LA, Casanova MF, Struble RG, Price DL: Phosphorylated neurofilament antigens in neurofibrillary tangles in Alzheimers disease. J Neuropath Exp Neurol 45:56-64, 1986.
Sternberger NH, Sternberger LA, Ulrich J: Aberrant neurofilament phosphorylation in Alzheimer disease. Proc Natl Acad Sci USA 82:4274-76, 1985.
Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - Neurofilament H Phosphorylated (SMI 34) Monoclonal Antibody
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Neurofilament H Phosphorylated (SMI 34) Monoclonal Antibody
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