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顺序:小鼠CD152 [CTLA-4] (氨基酸1-160)的胞外结构域融合在突变的小鼠IgG2a Fc区域的N端。
作用物种:小鼠。
生物活性:高亲和力结合CD80 (B7-1)和CD86 (B7-2),封闭CD80 (B7-1)和CD86 (B7-2)和他们的受体结合,竞争性抑制CD28信号通道从而阻止T细胞调控作用。显示CD152部分的生物活性。突变的Fc端,延长了半衰期。可用于研究T细胞共刺激。
内毒素含量:<0.06EU/μg蛋白 (LAL测试; Lonza)。重悬:用灭菌水重悬1mg/mL。用PBS稀释至需要的浓度。
形态:粉末,0.2uM过滤的PBS溶液。
保存:收到货后-20℃保存1年,工作液在-20℃可以保存3个月。
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文献和实验from their mothers ii. discard un-needed non-transgenic pups iii. house males and females separately f) when mice are 6 weeks old they may be mated (see 1. above) ES Cell-Mouse Chimera Breeding Based on our experience, we propose the following
Transgenic Mouse and Transgenic Rat Outline
-galactosidase or human placental alkaline phosphatase. You may use a non-mouse protein such as a human protein or an epitope tagged (i.e. HA, FLAG or myc) protein that can be identified with an antibody that doesn't bind to mouse proteins. For further discussion
Mouse Strains and Genetic Nomenclature
. We also provide some guidance to make the best possible choice when establishing an experimental protocol. Curr. Protoc. Mouse Biol. 1:213?238. © 2011 by John Wiley & Sons, Inc. Keywords: inbred strains; congenic strains; recombinant inbred
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