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顺序：小鼠CD152 [CTLA-4] (氨基酸38-160)的胞外结构域融合在小鼠IgG2a Fc区域的N端。
作用物种：小鼠。
生物活性：高亲和力结合CD80 (B7-1)和CD86 (B7-2)，竞争性抑制CD28信号通道。可几乎完全杀死靶细胞。
内毒素含量：<0.06EU/μg蛋白 (LAL测试; Lonza)。
重悬：用灭菌水重悬1mg/mL。用PBS稀释至需要的浓度。
形态：粉末，0.2uM过滤的PBS溶液。
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文献和实验Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
1) Resuspend the Dynabeads Mouse T-Activator CD3/CD28 in the vial. 2) Transfer the desired volume of Dynabeads to a tube. 3) Add an equal volume of Buffer, or at least 1 ml, and mix (vortex for 5 seconds
Blastocyst embryo transfer into pseudopregnant recipient female mouse（图）
. The Recipient Careful selection of the recipient is most important as the pups are the end result of a lot of hard work. I personally use Swiss Webster or CD-1 mice, as they are quiet and make excellent mothers, although do become overweight quickly
Th17 Polarization of Mouse CD4 Cells
实验试剂 Sterile PBS Cell culture medium Sterile plastic petridishes RBC Lysis Buffer [Details: Biolengend, cat.#420301] Anti-mouse CD3ε, clone145-2C11[Details: LEAF™format, cat.#100314]
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