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文献和实验Terminal Deoxynucleotidyl Transferase
Terminal Deoxynucleotidyl Transferase (TdT) is an enzyme that catalyzes the repetitive addition of mononucleotides from dNTPs to the terminal 3´-OH of a DNA initiator, accompanied by the release of inorganic phosphate. The enzyme
Radiolabeling of DNA with 3 Terminal Transferase
In contrast to the end labeling methods described in chapters 39 and chapters 41 , end labeling with terminal transf erase can be used for DNA fragments with 3′ protruding ends, 3′ recessed ends, and blunt ends. Terminal deoxynucleotidyl
Detection of Terminal Transferase in Leukemia
The mere presence of terminal deoxynucleotidyl transferase (TdT), a DNA polymerase, in leukemic cells provides no help in assigning these blast cells to a particular cell lineage (1 ). Differential levels of TdT gene transcription
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