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- 碧云天（beyotime）
- D2109
- 2025年08月15日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
1μg/100μg
| 规格： | 1μg | 产品价格： | ￥888.0 |
|---|---|---|---|
| 规格： | 100μg | 产品价格： | ￥1169.0 |
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| D2109-1μg | pAP1-TA-luc (报告基因质粒) | 1μg | 888.00元 |
| D2109-100μg | pAP1-TA-luc (报告基因质粒) | 100μg | 1169.00元 |
pAP1-TA-luc (报告基因质粒)是碧云天自行研发的用于检测AP1转录活性水平的报告基因质粒。pAP1-TA-luc是以碧云天的pGL6-TA质粒为模板，在其多克隆位点插入了多个AP1结合位点，可以高灵敏度地检测AP1的激活水平。
pGL6-TA质粒是用于在哺乳动物细胞中进行萤火虫萤光素酶(firefly luciferase)报告基因检测的新一代质粒。该报告基因质粒比Promega公司的pGL3系列有了全面的改进，一方面对于luciferase的编码进行了改进，确保能更好地在哺乳动物细胞中进行表达，同时对整个质粒中所有可以被预测出的可能的转录因子结合位点全部进行了适当的突变处理，在保持原有功能不变的情况下，使各种转录因子在质粒上的非特异性结合降到最低。
萤光素、萤光素酶、萤火虫萤光素酶和海肾萤光素酶也经常被称作荧光素、荧光素酶、萤火虫荧光素酶和海肾荧光素酶。
pAP1-TA-luc质粒的主要信息如下：
| Base pairs | 5653 |
| AP1 response element | 26-67 |
| Minimal TA promoter (pTA) | 90-112 |
| luc2 reporter gene | 154-1806 |
| SV40 late poly(A) signal | 1841-2062 |
| SV40 early enhancer/promoter | 2110-2528 |
| Synthetic neomycin phosphotransferase | |
| (Neor) coding region | 2553-3347 |
| Synthetic poly(A) signal | 3372-3420 |
| Reporter Vector primer 4 (RVprimer4) binding region | 3487-3506 |
| ColE1-derived plasmid replication origin | 3744 |
| Synthetic Beta-lactamase (Ampr) coding region | 4535-5395 |
| Synthetic poly(A) signal/transcriptional pause site | 5500-5653 |
| Reporter Vector primer 3 (RVprimer3) binding region | 5602-5621 |
pAP1-TA-luc质粒的图谱如下：
pAP1-TA-luc的多克隆位点及AP1 response element的详细图谱见说明书：www.beyotime.com/Manual/D2109 pAP1-TA-luc (报告基因质粒).pdf
pAP1-TA-luc中没有的酶切位点(Restriction enzymes that do not cut pAP1-TA-luc)包括：
| Aat II | Afl II | Asc I | Ase I | Bsa I | BsaA I | BsiW I | BspM II |
| BssH II | Eco72 I | EcoR I | EcoR V | Mlu I | Nde I | Nru I | PaeR7 I |
| PflM I | Pme I | Pml I | Psp1406 I | PspA I | Rsr II | Sac I | Sma I |
| SnaB I | Spl I | Srf I | Tth111 I | Vsp I | Xcm I | Xho I | Xma I |
pAP1-TA-luc中的单酶切位点(Restriction enzymes that cut pAP1-TA-luc once)包括：
| Sfi I | GGCCN,NNN`NGGCC | 9 | BstB I | TT`CG,AA | 3423 | ||
| Bgl | IGCCN,NNN`NGGC | 9 | Sal I | G`TCGA,C | 3437 | ||
| Acc65 I | G`GTAC,C | 15 | Afl III | A`CRYG,T | 3687 | ||
| Asp718 | G`GTAC,C | 15 | ApaL I | G`TGCA,C | 4001 | ||
| Kpn I | G,GTAC`C | 19 | HgiE II | ACCNNNNNNGGT -1/13 | 4266 | ||
| Nhe I | G`CTAG,C | 21 | Not I | GC`GGCC,GC | 4507 | ||
| Bgl II | A`GATC,T | 69 | BstX I | CCAN,NNNN`NTGG | 4531 | ||
| Hind III | A`AGCT,T | 79 | BstE II | G`GTNAC,C | 4534 | ||
| BsrG I | T`GTAC,A | 645 | Ahd I | GACNN,N`NNGTC | 4609 | ||
| Dra III | CAC,NNN`GTG | 1301 | Bsu36 I | CC`TNA,GG | 4965 | ||
| Gsu I | CTGGAG 21/19 | 1534 | Pvu I | CG,AT`CG | 4979 | ||
| Bpm I | CTGGAG 22/20 | 1535 | Sac II | CC,GC`GG | 5003 | ||
| Apo I | R`AATT,Y | 1917 | Bst1107 I | GTA|TAC | 5119 | ||
| Mun I | C`AATT,G | 1981 | Xca I | GTA|TAC | 5119 | ||
| BamH I | G`GATC,C | 2074 | Spe I | A`CTAG,T | 5438 | ||
| Stu I | AGG|CCT | 2507 | BsmA I | GTCTC`/9 | 5450 | ||
| EcoN I | CCTNN`N,NNAGG | 3028 | BsmB I | CGTCTC 7/11 | 5451 | ||
| BsiC I | TT`CG,AA | 3423 |
pAP1-TA-luc质粒中推荐使用的测序引物序列如下：
RVprimer3 (5602-5621):
CTA GCA AAA TAG GCT GTC CC
pAP1-TA-luc的全序列信息：d2109-pAP1-TA-luc-seq.txt
包装清单：
| 产品编号 | 产品名称 | 包装 |
| D2109 | pAP1-TA-luc (报告基因质粒) | 1μg |
| — | 说明书 | 1份 |
保存条件：
-20℃保存。
注意事项：
本质粒未经碧云天书面许可不得用于任何商业用途，也不得移交给订货人所在实验室外的任何个人或单位。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
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2. Hou R, Han Y, Fei Q, Gao Y, Qi R, Cai R, Qi Y . Dietary Flavone Tectochrysin Exerts Anti-Inflammatory Action by Directly Inhibiting MEK1/2 in LPS-Primed Macrophages. Mol Nutr Food Res. 2018 Jan;62(2). (IF 5.309)
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