- ￥553 - 4153
- 翌圣生物（Yeasen）
- 10607ES
- 上海翌圣
- 2026年10月19日
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- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
有效期1年
- 英文名：
Deoxyribonuclease I (DNase I) from bovine pancreas
- 供应商：
翌圣生物科技(上海)股份有限公司
- 库存：
大量
- 规格：
15 KU(10607ES15)/10× 15 KU(10607ES81)
| 规格： | 15 KU(10607ES15) | 产品价格： | ￥553.0 |
|---|---|---|---|
| 规格： | 10× 15 KU(10607ES81) | 产品价格： | ￥4153.0 |
产品描述：
脱氧核糖核酸酶I（DNase I），即Deoxyribonuclease I，一种发现于多种细胞和组织的核酸内切酶，靶向切割邻近嘧啶的磷酸二酯键，产生5’端为磷酸基团、3’端为羟基的多聚核苷酸，平均消化产物最小为多聚四核苷酸。DNase可催化多种形式DNA，如单链DNA、双链DNA，甚至染色质（其切割速率受组蛋白影响）。优选的工作范围是pH7-8，DNase的活性依赖于Ca2+，并可被二价金属离子如Co2+，Mn2+，Zn2+等激活。5 mM Ca2+可保护酶使其不被水解。在Mg2+存在下，该酶可随机识别和切断DNA任一条链上的任意位点；而在Mn2+存在的条件下，可同时识别DNA的两条链并在几乎相同的位点进行切割。DNase I最早从胰腺中分离而来，至今哺乳动物胰腺也是该酶的最主要的来源之一。
本品来自牛胰腺，由四种色谱区分的组分A，B，C，D构成，摩尔比为4:1:1，而D组分非常少。分子生物学实验中常用于清除蛋白中的DNA，或者用于向DNA中引入缺口使标记碱基插入DNA。本品以含氯化钙的冻干粉形式供应，酶活力≥2000 Kunitz Units/mg 蛋白。
产品特色：
| CAS号（CAS NO.） | 9003-98-9 |
| 分子量（Molecular Weight） | ~31 kDa |
| 孔尼茨单位（Kunitz Units） | ≥2000 Kunitz Units/mg protein |
| 类型（Type） | Type IV |
| 优选pH（Optimal pH） | 7～8 |
| 外观（Appearance） | 白色至浅褐色冻干粉 |
| 纯度（Purity） | Protein:≥80% by Biuret |
| 溶解性（Solubility） | 0.15 M NaCl：5 mg/mL，无色透明溶液 |
| 激活剂（Activators） | 多种二价金属离子如Mg2+、Mn2+, Ca2+, Co2+, and Zn2+ |
| 抑制剂（Inhibitors） | β-巯基乙醇；螯合剂；SDS；肌动蛋白 |
| 活力单位定义（Unit Definition） | 25℃，pH5.0条件下DNase催化底物DNA，使每毫升每分钟ΔA260增加0.001的变化定义为一个酶活力单位（Kunitz unit）。 |
应用案例：
| 推荐货号 | 产品名称 | 产品形态 | 浓度 | 来源 | 相互关系 | 使用建议/说明 |
| 10607ES | Deoxyribonuclease I (DNase I) from bovine pancreas 脱氧核糖核酸酶I，来源于牛胰腺 | 冻干粉 | ≥2000 Kunitz U/mg | 牛胰腺 | 与 10608 同牛胰腺来源，按质量（酶活单位）规格供货 | RNA 提取时去除 gDNA），适合于大部分核酸提取，不适合于微量RNA提取；植物组织RNA提取常用 |
| 10608ES | Deoxyribonuclease I (DNase I) from bovine pancreas 脱氧核糖核酸酶I，来源于牛胰腺 | 冻干粉 | ≥2000 Kunitz U/mg | 牛胰腺 | 与 10607 同牛胰腺来源，按质量（mg）规格供货 | 同 10607，适合按质量称量使用的场景；植物组织RNA提取常用 |
| 14549ES | Recombinant DNase I (RNase-free, Yeast) 重组DNase I (酵母表达)(5 U/μL) | 液体 | 5 U/μL | 酵母表达 | 无动物源、无 RNase 残留，残留控制比10325更严格 | RNA 提取 / 建库中去除 DNA，也适应于微量RNA提取，动物组织细胞RNA提取常用 |
| 10325ES | Recombinant DNase I (RNase-free) 重组DNase I (大肠杆菌表达) | 液体（重组） | 2 U/μL | 大肠杆菌表达 | 无动物源、无 RNase 残留 | RNA 提取 |
储存条件：
组分-25~-15℃保存，有效期2年；
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by bivalent metals. Maximum activation is attained with Mg+ and Ca+. Dnase I is inhibited by EDTA and SDS. The optimum pH of Deoxyribonuclease I from Bovine Pancreas is 7.8. 2.1.4. What is an acceptable enzyme diluent for Elastase (ES)? Elastase
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