
人冠状动脉平滑肌细胞
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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Human Coronary Artery Smooth Muscle Cells
- 细胞类型:
人正常原代细胞
- 物种来源:
人源
- 器官来源:
人源
- 运输方式:
干冰或液氮
- 年限:
液氮储存10年以上
- 生长状态:
冻存
- 规格:
500,000 cells/vial
Lifeline® normal Human Coronary Artery Smooth Muscle Cells (HCASMC), when grown in VascuLife® SMC Medium, provide an ideal low serum (5%) culture model for the study of angiogenesis, atherosclerosis, diabetes or vascular/pulmonary biology.
Lifeline® Coronary Artery Smooth Muscle Cells have been isolated from human coronary arteries, plated and expanded in culture vessels twice before being harvested for cryopreservation to ensure the highest purity, viability and plating efficiency.
Coronary artery smooth muscle cells, 100X
Our Human Smooth Muscle Cells are quality tested in VascuLife® SMC Medium and demonstrate optimal low serum growth over a period of at least 15 population doublings at rates equal to or greater than other serum-supplemented media.
Lifeline® coronary artery smooth muscle cells are not exposed to antimicrobials or phenol red when cultured in VascuLife® SMC Medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results. Lifeline® offers these traditional supplements; however, they are not needed, or recommended, to achieve optimal cell performance in most research applications.
- Coronary artery smooth muscle cells can be grown without phenol red or antimicrobials when cultured in VascuLife® SMC Medium.
500,000 cells per vial
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文献和实验一、摘要 目的:建立兔膀胱平滑肌细胞的分离、培养和鉴定的方法。方法:成年新西兰白兔两只(正常及梗阻各一只),采用酶法分离技术获得膀胱平滑肌细胞后于10%小牛血清的DMEM中培养,观察细胞形态和扩增情况,用爬片染色、电镜、蛋白质α-肌动蛋白(α-actin)鉴定细胞类型。结果:倒置显微镜下观察均呈“谷和峰”样结构、细胞爬片HE染色及电镜检查均证实为平滑肌细胞。免疫组化染色检测α-actin呈阳性反应。从细胞爬片HE染色和免疫组化染色检测α-actin呈阳性反应中我们发现该方法所的膀胱平滑肌细胞
用动脉夹夹住血管两侧,提前穿好结扎线,用1毫升注射器刺破血管,分离组织股动脉血管,股动脉插入球囊,球囊内注入空气,一分钟后将球囊拔出,缝合血管,此方法的主要操作是将一定直径大小的球囊放入动脉血管后充盈球囊,球囊内注入空气,通扩充球囊造成动脉血管内皮细胞损伤,弹力板及中膜严重损伤,引起局部血管狭窄。进行球囊损伤手术时需要注意选择内径大小适宜的球囊,牵拉过程中速度、力度的把握以及对球囊扩张压力的控制。
佚名 冠状动脉性心脏病(coronary heart disease),简称冠心病,是指因狭窄性冠状动脉疾病而引起的心肌缺氧(供血不足)所造成的缺血性心脏病。冠心病绝大多数由冠状动脉粥样硬化引起。引起冠心病的原因: 1.冠状动脉粥样硬化 为最常见的狭窄性冠状动脉疾病,特别是肌壁外冠状动脉支的动脉粥样硬化。冠状动脉近侧段之所以好发
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