Bst2BI内切酶价格

特别提示：包括Bst2BI内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：Bst2BI内切酶
英文名称：Bst2BI
产品货号：GS2240
产品规格：100U

储存条件：冷冻（-20℃）

使用说明

To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1hourat 60℃ in a total reaction volume of 50μl.
Recognition sites	C↑TCGTG
来源	Bacillus stearothermophilus 2B
Assayed on	Lambda DNA
Optimal buffer	Y(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.)+ BSA
Optimal temperature	60℃
Storage conditions	10 mM Tris-HCl(pH 7.5);200 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;50%glycerol.Store at -20℃.
Ligations	After 10-fold overdigestion with enzyme 95%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a.of enzyme for 16hoursat 60℃.
Methlation effect	Methylation sensitivity not tested
Thermal inactivation	enzyme is inactivated by incubation at 80℃ for 20 mins.
Campatible Ends	BauI,BsiI,BssSI

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