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- 详细信息
- 文献和实验
- 技术资料
- 库存:
48
- 英文名:
Nonesterified Free fatty acids assay kit
- 保质期:
详见说明书
- 供应商:
上海一研
- 保存条件:
低温
- 规格:
50管/48样
英文名称:Nonesterified Free fatty acids assay kit
产品规格:50管/48样
发货周期:1~3天
检测指标:游离脂肪酸;NEFA
本试剂盒可测各种动物血清、血浆、组织等样本中游离脂肪酸的含量。游离脂肪酸,别名非酯化脂肪酸,是脂肪水解产物,测定血清脂肪酸可以了解脂肪代谢的情况,升高代表脂肪分解增加。游离脂肪酸能与铜离子结合形成脂肪酸的铜盐而溶于氯仿中,其含量与游离脂肪酸含量成正比。用铜试剂测定其中铜离子的含量,即可推算出游离脂肪酸的含量。
优点如下:
1、快速简便:全程约2小时,可测50例左右样本。
3、再现性好:变异系数CV=1.5%。
4、回收试验: X =96%。
5、受外界影响因素小:干扰因素少,重复性强。
6、测试面广:可测各种动物血清(浆)、组织,效果均佳。
游离脂肪酸测定试剂盒(比色法) 使用注意事项
1. 微量试剂取用前请离心集液。
2. Annexin V-EGFP,Propidium Iodide(PI)避光保存及使用。
3. Propidium Iodide (PI)有毒,操作时请戴手套。
4. 本试剂盒适用于检测活细胞,流式细胞仪检测时,细胞数量不应低于1×105,,不推荐用于检测组织样本。
5. 推荐使用悬浮培养细胞。如果是贴壁细胞,需用不含EDTA的胰酶消化,如消化不当,可能引起假阳性,而用细胞刮子会造成细胞粘连成团,而影响检测。可将胰酶消化后细胞的保存在含2%BSA的PBS中,防止进一步的损伤。
6. 细胞固定后可能导致荧光的淬灭,请不要固定样品。
7. 因检测细胞的类型、凋亡诱导剂种类、使用的检测仪器不同,因而流式检测的荧光补偿也不同,因此建议每次检测均需使用未经凋亡诱导处理的细胞作为对照,进行荧光补偿的调节。
Fonsecaea pedrosoi佩氏着色霉探针法荧光定量PCR试剂盒别 名 Developmentally regulated GTP binding protein 1; Developmentally-regulated GTP-binding protein 1; DRG 1; DRG-1; DRG1; DRG1_HUMAN; NEDD 3; NEDD-3; NEDD3; Neural precursor cell expressed developmentally down regulated protein 3; Neural precursor cell expressed developmentally down-regulated protein 3.免 疫 原 KLH conjugated synthetic peptide derived from human DRG1产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Fonsecaea spp.着色霉PCR试剂盒别 名 Differentially expressed in adenocarcinoma of the lung protein 1; 4.1B; Band 4.1 like protein 3; DAL 1; DAL1; EPB41L3; Erythrocyte membrane protein band 4.1 like 3; HGNC:3380; KIAA0987; E41L3_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human DAL1/EPB41L3产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Fonsecaea spp.着色霉染料法荧光定量PCR试剂盒别 名 Degenerative spermatocyte homolog 1; Degenerative spermatocyte homolog 1 lipid desaturase; Degenerative spermatocyte homolog lipid desaturase; DEGS 1; DEGS; Des 1; DES1; Dihydroceramide desaturase; FADS7; Membrane fatty acid (lipid) desaturase; Membrane lipid desaturase; MGC5079; MIG15v Migration inducing gene 15 protein; MLD; Sphingolipid delta 4 desaturase; Sphingolipid delta(4) desaturase DES1.DEGS1_HUMAN免 疫 原 KLH conjugated synthetic peptide derived from human DEGS1产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Fonsecaea spp.着色霉探针法荧光定量PCR试剂盒别 名 7 dehydrocholesterol reductase; 7 DHC reductase; Delta 7 dehydrocholesterol reductase; Putative sterol reductase SR 2; Sterol Delta; DHCR7_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human DHCR7产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Foot-and-Mouth Disease Virus(FMDV)口蹄疫病毒Asia1亚型RT-PCR试剂盒别 名 DAP kinase related apoptosis inducing protein; DAP kinase related apoptosis inducing protein kinase 2; Death associated protein kinase related 2; DRAK 2; Serine/threonine kinase 17b (apoptosis inducing); Serine/threonine kinase 17b; Serine/threonine protein kinase 17B; STK17B; ST17B_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human DRAK2产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Foot-and-Mouth Disease Virus(FMDV)口蹄疫病毒Asia1亚型染料法荧光定量RT-PCR试剂盒别 名 DEN2D_HUMAN; DENN domain-containing protein 2D; DENN/MADD domain containing 2D; DENND2D.免 疫 原 KLH conjugated synthetic peptide derived from human DENND2D产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Foot-and-Mouth Disease Virus(FMDV)口蹄疫病毒Asia1亚型探针法荧光定量RT-PCR试剂盒别 名 Type 3 DI; D3; 5DIII; Deiodinase iodothyronine type 3; Deiodinase iodothyronine type III; Dio 3; DIOIII; MGC124117; MGC124118; TXDI3; Type III 5' deiodinase; Type III iodothyronine deiodinase.免 疫 原 KLH conjugated synthetic peptide derived from human Dio3 (65-106aa)产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Foot-and-Mouth Disease Virus(FMDV)口蹄疫病毒A亚型RT-PCR试剂盒别 名 DKFZp781A0112; DNA polymerase eta; DNA polymerase theta; DPOLQ_HUMAN; POLH; POLQ; Polymerase (DNA directed) theta; PRO0327.免 疫 原 KLH conjugated synthetic peptide derived from human DNA Polymerase theta/POLH产品应用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Foot-and-Mouth Disease Virus(FMDV)口蹄疫病毒A亚型染料法荧光定量RT-PCR试剂盒别 名 Dilute suppressor; Dilute suppressor protein homolog; DSU; Melanoregulin; MGC90296; MREG; WDT 2; WDT2; Whn dependent transcript 2; MREG_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human DSU/MREG产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Foot-and-Mouth Disease Virus(FMDV)口蹄疫病毒A亚型探针法荧光定量RT-PCR试剂盒别 名 DAG kinase epsilon; DAGK 5; DAGK 6; DAGK5; DAGK6; DGK; DGK epsilon; DGK-epsilon; DGKE; DGKE_HUMAN; Diacylglycerol kinase epsilon 64kDa; Diacylglycerol kinase epsilon; Diglyceride kinase epsilon.免 疫 原 KLH conjugated synthetic peptide derived from human DGKE产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Foot-and-Mouth Disease Virus(FMDV)口蹄疫病毒C亚型RT-PCR试剂盒别 名 Di Ras1; DIRAS family GTP binding RAS like 1; Distinct subgroup of the Ras family member 1; GBTS1; GTP binding protein Di Ras1; Ras related inhibitor of cell growth; RIG; Rig protein; Small GTP binding tumor suppressor 1.免 疫 原 KLH conjugated synthetic peptide derived from human DIRAS1产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Foot-and-Mouth Disease Virus(FMDV)口蹄疫病毒C亚型染料法荧光定量RT-PCR试剂盒别 名 DD83.1; Dehydrogenase/reductase (SDR family) member 3; EC 1.1.1.300; MGC125166; RDH17; Retinal short chain dehydrogenase/reductase 1; retSDR1; Rsdr1 antibody SDR family, member 3; SDR1; SDR16C1; Short-chain dehydrogenase/reductase 3; Short-chain dehydrogenase/reductase family, member 3.免 疫 原 KLH conjugated synthetic peptide derived from human DHRS3 (111-160aa)产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Foot-and-Mouth Disease Virus(FMDV)口蹄疫病毒C亚型探针法荧光定量RT-PCR试剂盒别 名 Damage regulated autophagy modulator; Damage-regulated autophagy modulator; DNA damage-regulated autophagy modulator protein 1; dram1; DRAM1_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human DRAM产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Foot-and-Mouth Disease Virus(FMDV)口蹄疫病毒O亚型RT-PCR试剂盒别 名 CAGE; Cancer associated gene protein; Cancer/testis antigen 26; CT26; DEAD (Asp Glu Ala Asp) box polypeptide 53; DEAD box protein 53; DEAD box protein CAGE; Probable ATP dependent RNA helicase DDX53; DDX53_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human DDX53/CT26 (245-290aa)产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Foot-and-Mouth Disease Virus(FMDV)口蹄疫病毒O亚型染料法荧光定量RT-PCR试剂盒别 名 Cell cycle control protein SDP35; DEP domain containing 1; DEP domain containing protein 1A; DEP domain-containing protein 1A; DEP.8; DEP1A_HUMAN; DEPDC 1; DEPDC 1A; DEPDC1; DEPDC1-V2; Depdc1a; FLJ20354; SDP 35; SDP35.免 疫 原 KLH conjugated synthetic peptide derived from human DEPDC1 (606-660aa)产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
TIMP-1(CT) 金属蛋白酶组织抑制因子-1抗体(C端)Rhesus antibody Rh规格 0.1ml
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TIM4 T淋巴细胞膜蛋白4抗体Rhesus antibody Rh规格 0.1ml
TIGAR TIGAR蛋白抗体Rhesus antibody Rh规格 0.1ml
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DRG1 GTP发育调控结合蛋白1抗体进口/原装小鼠基质金属蛋白酶抑制因子2(TIMP-2)ELISA Kit,48T/96T
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DNAJB4/HSP40 homolog 热休克蛋白家族40抗体进口/分装大鼠基质金属蛋白酶抑制因子2(TIMP-2)ELISA Kit,48T/96T
粘蛋白17检测试剂盒中药对照药材特价促销七氟烷相关物质B标准品七氟烷相关物质B标准品48T/96T特价供应规格:
DAL1/EPB41L3 细胞骨架4.1蛋白家族DAL1抗体特价促销人基质金属蛋白酶抑制因子3(TIMP-3)ELISA Kit,48T/96T
粘蛋白2检测试剂盒中药对照药材进口/国产钠曲相关物质A标准品钠曲相关物质A标准品48T/96T现货供应规格:
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文献和实验[基本原理] MTT法是Mosmann 1983年报道的,以后此方法得到了迅速发展并广泛应用于临床与科研研究。其原理是活细胞内线粒体脱氢酶能将四氮唑化合物(MTT)由黄色还原为蓝黑色的甲肷,甲肷的生成量与细胞的增殖程度呈正相关。后者溶于有机溶剂(如二甲亚枫或酸化异丙醇等),并可在560nm波长下进行检测。 [试剂及材料] (1)MTT:用PBS配成5mg/ml的存储液,过滤除菌,4℃保存。 (2) 溶剂:二甲
[基本原理] XTT比色法有Scudiero等首次采用,用于检测细胞增殖。XTT是异种与MTT类似的四唑氮衍生物,可被活细胞线粒体脱氢酶还原成水溶性的棕色甲肷产物,当XTT与电子偶合剂共同使用时,甲肷的生成量与细胞的增殖程度呈正相关。 [试剂及材料] (1)XTT:用60℃预热培养液配制成6.6mmol/L,过滤除菌,现配现用。 (2)吩嗪二甲酯硫酸盐(PMS):用PBS配制成220mmol/L,4℃避光保存20天。 (3) XTT/PMS:XTT与PMS
一、目的植物体内的还原糖主要是葡萄糖、果糖和麦芽糖。它们在植物体内的分布,不仅反映植物体内碳水化合物的运转情况,而且也是合成其它成分碳架来源和呼吸作用的基质。此外,水果、蔬菜中含糖量的多少,也是鉴定其品质的重要指标。其它碳水化合物,如淀粉、蔗糖等,经水解也生成还原糖。因此,测定还原糖的方法在研究植物体内生理生化变化和测定植物体内碳水化合物方面都是很重要的。二、原理还原糖是具有羰基(>C=O)的糖,能将其它物质还原而其本身被氧化。(1) 还原糖在碱性条件及有酒石酸钾钠存在下加热,可以定量地还原
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