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文献和实验E.Z.N.A.® HP Total RNA Kit Spin Protocol Eukaryotic Cells and Tissues
to an Rase-Free syringe. Proceed to Step 3. 3. Transfer the lysate into a gDNA Removal Column placed in 2 ml collection tube. Centrifuge at 14,000 x g for 3 minutes at room temperature. Transfer the flow-through lysate into a new 1.5 ml tube. Proceed
Co-Expression of human DNA primase p49-p58 subunits
Adapted from W.C.Copeland,Protein Exp.& Purif 9,(1997)1-9 and Schneider,et.al.,JBC 273:34 (1998)21608-21615. Obtained from Fanning lab and revised by LSM,7/23/01 Obtained cell stock of BL21(DE3)cells cotransformed with pET21a-Hp49 (ampicillin
配制 (1)吖啶橙浓缩液(4℃、避光,可保存数月) 双蒸水 50ml 吖啶橙 50mg (2)甲液 双蒸水 200ml HCl 16ml NaCl 1.74g Triton X-100 0.2ml (3)乙液 0.2mol/LNa2HP04/0.1mol/L枸橼酸缓冲液(pH 6.0) 双蒸水 100ml Na2 HP04・12H20
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