MboII内切酶价格

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产品名称：MboII内切酶
英文名称：MboII
产品货号：GS2219
产品规格：100U

储存条件：冷冻（-20℃）

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA(dam-)in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sites	GAAGA(N)8↑
来源	E.coli strain,that carries the cloned gene Mbo II from Moraxella bovis
Assayed on	Lambda DNA(dam-)
Optimal buffer	Y?(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.)
Optimal temperature	37℃
Storage conditions	10 mM Tris-HCl(pH 7.5);50 mM NaCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;50%glycerol.Store at -20℃.
Ligations	After 5-fold overdigestion with enzyme more than 60%of the DNA fragments can be ligated and recut.In presence of 10%PEG ligation is better.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 5 u.a.of enzyme for 16hoursat 37℃.
Methlation effect	Blocked by overlappin dam-methylation(GmATC):?GAAGATC.
Thermal inactivation	enzyme is inactivated by incubation at 65℃ for 20 mins.

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