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- 详细信息
- 文献和实验
- 技术资料
- 库存:
194
- 英文名:
Recombinant Human Stromal-Cell Derived Factor-1 beta/CXCL12β
- 保质期:
长期
- 供应商:
北京百奥莱博科技有限公司
- 保存条件:
冷冻(-20℃)
- 规格:
2μg
特别提示:包括重组人SDF-1β/CXCL12β在内,本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
产品名称:重组人SDF-1β/CXCL12β
英文名称:Recombinant Human Stromal-Cell Derived Factor-1 beta/CXCL12β
产品货号:GS4527
产品规格:2μg
分子量:Approximately 8.5kDa,a single non-glycosylated polypeptide chain containing 72 amino acid residues.
储存条件:冷冻(-20℃)
概述
CXCL12 also known as SDF-1 is belonging to the CXC chemokine family.It is encoded by the CXCL12 gene.In recently study,Human CXCL12 is expressed as six isoforms that differ only in the C-terminal tail.And all SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids.In all SDF-1 isoforms,SDF-1β is the canonical sequence.It has the complete amino acids in the C-terminal tail.On the cell surface,the receptor for this chemokine is CXCR4 and syndecan4.CXCL12 is strongly chemotactic for T-lymphocytes,monocytes,but not neutrophils.CXCL12 is a very important factor in carcinogenesis and the neovascularisation linked to tumor progression.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | >97%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Fully biologically active when compared to standard.The biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 20-80ng/ml. |
| 成分 | Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4. |
| 内毒素 | Less than 1 EU/μg of rHuSDF-1β/CXCL12β as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNKRF KM |
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文献和实验:79 4,杨化新,张培培,徐康森.重组人生长激素胰肽图谱分析.药物分析杂志,1994,14(3):10 5,张培培,杨化新,徐康森.胰岛素的HPLC肽图谱分析.药物生物技术,1996,3(2):91 6,Geng X,Chang XJ.High performance hydrophobic interaction chromatography as a tool for protein refolding. Chromatogr,1992,599,185 7,Kunitani M
=0 [求助]Western Blot检测脑组织GABAA、NMDA、ACH受体亚型的方法 http://www.dxy.cn/bbs/post/view?bid=65&id=5464047&sty=1&tpg=19&age=0 [求助]首次采用脾内免疫,第二次加强用完全佐剂还是用不完全佐剂好呢? http://www.dxy.cn/bbs/post/view?bid=65&id=5465370&sty=1&tpg=19&age=0 [求助]北京哪个公司单抗N端测序列做的好 http
羊抗鼠IgG,北京),放入37℃2h后,用PBS洗涤3次。然后立即在荧光显微镜下观察,摄片。 血管内皮细胞鉴定结果: VWF相关抗原间接免疫荧光检查,原代及传代培养的内皮细胞浆中有黄绿色的荧光着色,胞核呈黑绿色,整个细胞轮廓清楚。作为对照的内皮细胞片子中,偶见绿色斑点散发荧光,未见细胞轮廓。 5.内皮细胞体外生长情况 用胰蛋白酶消化的人脐静脉内皮细胞,接种在24孔塑料培养板各孔内4h后,大部分细胞贴壁。早期细胞呈小多角、球形、呈团状,少数细胞伸展,48~72h生长最快,逐渐生长成梭形,有些细胞
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