BstV1I内切酶报价

特别提示：包括BstV1I内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：BstV1I内切酶
英文名称：BstV1I
产品货号：GS2206
产品规格：50U

储存条件：冷冻（-20℃）

使用说明

At 37℃ activity is 10%from maximum.

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1μgof pBR322 DNA in 1hourat 55℃ in a total reaction volume of 50μl.
Recognition sites	GCAGC(N)8↑
来源	Bacillus stearothermophilus V1
Assayed on	pBR322 DNA
Optimal buffer	G?(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;50 mM NaCl;1 mM DTT.)
Optimal temperature	55℃
Storage conditions	10mMTris-HCl(pH 7.5);200 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;50%glycerol.Store at -20℃.
Ligations	After 3-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1μgof DNA with 2 u.a.of enzyme for 16hoursat 55℃.
Methlation effect	Methylation sensitivity not tested
Thermal inactivation	enzyme is inactivated by incubation at 80℃ for 20 mins.
Campatible Ends	BbvI,BseXI,Bst71I,R9896

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