- 询价
- Cell Signaling Technology
- 5873
- USA
- 2026年01月09日
- IP
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Mouse IgG (Magnetic Bead Conjugate)
- 应用范围：
IP
- 宿主：
Mouse
- 库存：
大量
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 供应商：
CST
- 标记物：
Magnetic Bead
- 是否单克隆：
0
- 保存条件：
-20°c
- 规格：
400 ul/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 400 ul | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: IP=Immunoprecipitation
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Source |
|---|---|---|
| IP | Mouse |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Normal mouse IgG is not expected to recognize any specific antigen. |
| Source / Purification | Normal mouse IgG was isolated by affinity chromatography from naive mouse serum. IP
Immunoprecipitation using NIH/3T3 (lane 1 and 2) and C6 (lane 3 and 4) cell extracts using Mouse IgG (Magnetic Bead Conjugate) (lane 1 and 3) or Akt (pan) (40D4) Mouse mAb (Magnetic Bead Conjugate) #5697 (lane 2 and 4). The western blot was probed using Akt (pan) (C67E7) Rabbit mAb #4691. |
| Description | This Cell Signaling Technology normal mouse IgG (whole molecule) containing predominantly IgG1 and IgG2a isotypes and small amounts of IgG2b and IgG3 is immobilized by the covalent reaction of hydrazinonicotinamide-modifed antibody with formylbenzamide-modified magnetic beads. Mouse IgG (Magnetic Bead Conjugate) is useful to determine non-specific immunoprecipitation complexes. |
| Directions for Use | Add 10 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) #9803. See protocol for more details. For bead washing and subsequent elution of immunocomplexes, the beads can be separated from solution using our 6-Tube Magnetic Separation Rack #7017. Place the tubes containing the beads in the Magnetic Separation Rack and wait 1 to 2 minutes for the solution to clear before carefully removing the supernatant. Remove the tubes from the Magnetic Separation Rack, add new solution and resuspend the beads by gently vortexing or rocking the tube. |
| Background | Control antibodies are used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验a sample tube for each fluorochrome-conjugated mouse antibody you are using, and add 1 drop of AbC™ capture beads (Component A) to each tube. 3) Add a pre-titrated amount of each mouse antibody conjugate to the AbC™ capture bead suspension
Mouse Morphological Phenotyping With Magnetic Resonance Imaging
The field of mouse phenotyping with magnetic resonance imaging (MRI) is rapidly growing, with both MRI physicists and biologists starting to use MRI to identify mouse models of human disease. The purpose of this chapter is to provide details
Magnetic Resonance Microscopy of Mouse Brain Development
Magnetic resonance imaging (MRI) is increasingly becoming an important tool to study anatomy of rodent brains. Compared with histology, it has clear advantages because the entire 3D object can be captured as an image nondestructively
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