- 询价
- Cell Signaling Technology
- 3420
- USA
- 2026年05月29日
- IP
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Mouse IgG (Sepharose Bead Conjugate)
- 应用范围：
IP
- 供应商：
CST
- 级别：
详见MSDS文件
- 库存：
大量
- 保质期：
详见说明书
- 标记物：
Sepharose Bead
- 是否单克隆：
0
- 保存条件：
-20°c
- 规格：
400 ul (40 immunoprecipitations)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 400 ul (40 immunoprecipitations) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
Product Pathways - Complementary Reagents
Mouse IgG (Sepharose Bead Conjugate) #3420
Applications
Immunoprecipitation
Protocols
- 3420:
- Immunoprecipitation
Description
This Cell Signaling Technology normal mouse IgG (whole molecule) containing predominantly IgG1 and IgG2a isotypes and small amounts of IgG2b and IgG3 is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Mouse IgG (Sepharose Bead Conjugate) is useful to determine non-specific immunoprecipitation complexes.
Specificity / Sensitivity
Normal mouse IgG is not expected to recognize any specific antigen.
Source / Purification
Normal mouse IgG was isolated by affinity chromatography from naive mouse serum.
IP
Western blot analysis of extracts from COS cells transfected with Myc-Chorionic Somatomammotropin Hormone-Like 1 (CSL) protein (lane 1). Immunoprecipitation of transfected COS lysates with Mouse IgG (Sepharose Bead Conjugate) (lane 2) or Myc-Tag (9B11) (Sepharose Bead Conjugate) Mouse mAb #3400 (lane 3). Blot was probed with Myc Tag (71D10) Rabbit mAb #2278.
Background
Control antibodies are used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验【原创】Protein A Sepharose 之进化篇。。。
的是经过GE填料洗脱后稀释不同浓度抗体。7号孔点的是上柱后穿流人血清。8号孔点的是上柱前人血清。9号孔点的是Marker。 从图中，我们可以看出，与GE公司rProtein A Sepharose FF填料从人血清纯化抗体纯度比较，Putus填料纯化的抗体纯度更高。更重要的是，后者的洗脱条件仅为4.5，高于前者的洗脱条件3.4。使用具备较少B结构域的Protein A柱能获得高纯度的IgG，并且洗脱条件温和，对蛋白质的损伤小。
a sample tube for each fluorochrome-conjugated mouse antibody you are using, and add 1 drop of AbC™ capture beads (Component A) to each tube. 3) Add a pre-titrated amount of each mouse antibody conjugate to the AbC™ capture bead suspension
相关专题 (一) 原理 葡萄球菌A蛋白(SPA)具有与多种哺乳动物 IgG分子Fc段结合的能力，并与不同IgG亚类的结合力有所差别。利用改变PH及离子强度可洗脱结合于SPA-Sepharose CL-4B柱上的IgG或不同的IgG亚类，可直接纯化血清或小鼠腹水中的IgG抗体。 (二) 操作步骤 用0.1M PH8.0 磷酸缓冲液 浸泡SPA-Sepharose CL-4B凝胶，15min。按1g干胶200ml
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