- 询价
- Cell Signaling Technology
- 3739
- USA
- 2026年05月29日
- F
- All
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- 文献和实验
- 技术资料
- 抗体英文名：
Myc-Tag (9B11) Mouse mAb (PE Conjugate)
- 抗原：
synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL)
- 应用范围：
F
- 适应物种：
All
- 级别：
详见MSDS文件
- 供应商：
CST
- 库存：
大量
- 保质期：
详见说明书
- 标记物：
PE
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (50 tests)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: F=Flow Cytometry
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| F | All | Transfected Only | Mouse IgG2a |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Myc-Tag (9B11) Mouse mAb (PE Conjugate) detects recombinant proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL). Flow Cytometry
Flow cytometric analysis of COS-7 cells, untreated (blue) or Myc transfected (green), using Myc-Tag (9B11) Mouse mAb (PE Conjugate). |
| Description | This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry in cells transfected with Myc-tagged protein. |
| Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验WHOLE MOUNT IN SITU HYBRIDIZATION. Mouse Embryos
4.5 (without removing prehybridization solution.) C. Repeat the addition of the 2XSSC wash twice more. Remove the mix and wash twice, 30 min each time, in 2XSSC pH7/0.1% CHAPS 70 °C. Wash twice, 10 min each, in Maleic Acid Buffer ( MAB; 100 mM maleic acid
Immunofluorescent Staining of Mouse and Rat Leukocytes
-up, please see description in "Procedure for Setting Compensation for Multi-Color Flow Cytometric Analysis". For reducing FcgII/IIIR-mediated antibody binding (or binding of SAv-PE or SAv-Cy-Chrome) which could contribute to background, the use of anti-mouse CD32/CD
WHOLE MOUNT IN SITU HYBRIDIZATION.Mouse Embryos
expressed in the anterior primitive endoderm of the mouse gastrula" Mechanism of Development, in press - 1997. Modified from the protocol of Harland (1991) and Current Protocols in Molecular Biology. Preparation of the probe.
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