- 询价
- Cell Signaling Technology
- 8179
- USA
- 2025年10月24日
- W, IHC-P, IF-IC
- H,M,R,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
HMGA2 (D1A7) Rabbit mAb
- 抗原：
synthetic peptide corresponding to residues near the amino terminus of human HMGA2 protein
- 应用范围：
W, IHC-P, IF-IC
- 级别：
详见MSDS文件
- 库存：
大量
- 保质期：
详见说明书
- 供应商：
CST
- 适应物种：
H,M,R,Mk
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC | H M R (Mk) | Endogenous | 18 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | HMGA2 (D1A7) Rabbit mAb recognizes endogenous levels of total HMGA2 protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human HMGA2 protein. Western Blotting
Western blot analysis of extracts from various cell lines using HMGA2 (D1A7) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using HMGA2 (D1A7) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded cell pellets, HCT 116 (left) or HBP-ALL (right), using HMGA2 (D1A7) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human uterine leiomyoma using HMGA2 (D1A7) Rabbit mAb. IF-IC
Confocal immunofluorescent analysis of A549 cells, untreated (left) or treated with hTGF-β1 #8915 (10 ng/ml) and hIL-1β #8900 (5 ng/ml, 48 hr; right), using HMGA2 (D1A7) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). |
| Background | HMGA2 belongs to the family of high mobility group with AT-hook DNA binding domain. HMGA proteins are considered architectural transcription factors; they do not have direct transcriptional activation capacity, but instead regulate gene expression by changing DNA conformation through binding to AT-rich regions in the DNA and/or direct interaction with other transcription factors (1,2). HMGA2 is abundantly and ubiquitously expressed and plays a crucial role during embryonic development (3). HMGA2 promotes stem cell self-renewal and research studies have shown that decreased HMGA2 expression is associated with stem cell aging (4-7). Investigators have shown that expression levels of HMGA2 are very low in normal adult tissues, while either overexpression or rearrangement is associated with many types of cancer (8-11).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Methods for the Detection of D-Amino-Acid Oxidase
then twice for 5 min. It was incubated for 1 hr in TBS-T containing rabbit anti-hog D-amino-acid oxidase IgG (1/3,000 dilution) and quickly rinsed twice with TBS-T and further washed in TBS-T once for 15 min then twice for 5 min. The membrane was incubated
组织化学分析。（图 C）使用Anti-Erk1/2 Mouse mAb 鼠单抗进行目的蛋白检测；(图 D) 使用p44/42 MAPK （Erk1/2）Rabbit mAb 兔单抗进行目的蛋白检测。 图 7 免疫组化实验检测Akt表达 注：使用 Anti-Akt (pan) Rabbit mAb 对正常小鼠肝组织进行免疫组织化学分析。（图 E）使用免疫组化试剂盒 M&R HRP/DAB Detection IHC Kit，抗体1:100 稀释；(图F)使用另一种试剂盒，抗体 1:100 稀释。
Methods for the Detection of D-Amino-Acid Oxidase
min then twice for 5 min. It was incubated for 1 hr in TBS-T containing rabbit anti-hog D-amino-acid oxidase IgG (1/3,000 dilution) and quickly rinsed twice with TBS-T and further washed in TBS-T once for 15 min then twice for 5 min. The membrane
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