Bax (D2E11) Rabbit mAb

5023:

IHC / Paraffin , Immunoprecipitation , Western Blotting

Bax (D2E11) Rabbit mAb detects endogenous levels of total Bax protein.

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu45 of human Bax protein.

Western Blotting

Western blot analysis of extracts from various cell lines using Bax (D2E11) Rabbit mAb. Brimmel et al. demonstated that Jurkat cells lack Bax protein expression (10).

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast caricnoma using Bax (D2E11) Rabbit mAb in the presence of control peptide (upper) or antigen-specific peptide (lower).

The Bcl-2 family consists of a number of evolutionarily conserved proteins containing Bcl-2 homology domains (BH) that regulate apoptosis through control of mitochondrial membrane permeability and release of cytochrome c (1-3). Four BH domains have been identified (BH1-4) that mediate protein interactions. The family can be separated into three groups based upon function and sequence homology: pro-survival members include Bcl-2, Bcl-xL, Mcl-1, A1 and Bcl-w; pro-apoptotic proteins include Bax, Bak and Bok, and "BH3 only" proteins Bad, Bik, Bid, Puma, Bim, Bmf, Noxa and Hrk. Interactions between death-promoting and death-suppressing Bcl-2 family members has led to a rheostat model in which the ratio of pro-apoptotic and anti-apoptotic proteins controls cell fate (4). Thus, pro-survival members exert their behavior by binding to and antagonizing death-promoting members. In general, the "BH3-only members" can bind to and antagonize the pro-survival proteins leading to increased apoptosis (5). While some redundancy of this system likely exists, tissue specificity, transcriptional and post-translational regulation of many of these family members can account for distinct physiological roles.

Bax is a key component for cellular induced apoptosis through mitochondrial stress (6). Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane (7). Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9 and initiation of the caspase activation pathway for apoptosis (8,9).

1. Cory, S. et al. (2003) Oncogene 22, 8590-607.
2. Antonsson, B. and Martinou, J.C. (2000) Exp Cell Res 256, 50-7.
3. Sharpe, J.C. et al. (2004) Biochim Biophys Acta 1644, 107-13.
4. Korsmeyer, S.J. et al. (1993) Semin Cancer Biol 4, 327-32.
5. Bouillet, P. and Strasser, A. (2002) J Cell Sci 115, 1567-74.
6. Wei, M.C. et al. (2001) Science 292, 727-30.
7. Jürgensmeier, J.M. et al. (1998) Proc Natl Acad Sci USA 95, 4997-5002.
8. Narita, M. et al. (1998) Proc Natl Acad Sci USA 95, 14681-6.
9. Marzo, I. et al. (1998) Science 281, 2027-31.
10. Brimmell, M. et al. (1998) Oncogene 16, 1803-12.

• Kim, K.D. et al. (2011) J Immunol 187, 3620-30. Applications: Western Blotting
• Weisberg, E. et al. (2011) PLoS One 6, e25351. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

• 2772 Bax Antibody
• 2774 Bax Antibody (Human Specific)
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 8112 SignalStain^® Antibody Diluent
• 8114 SignalStain^® Boost IHC Detection Reagent (HRP, Rabbit)

For Research Use Only. Not For Use In Diagnostic Procedures.