- 询价
- Cell Signaling Technology
- 4006
- USA
- 2025年07月16日
- W
- Rabbit
- R
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-Na,K-ATPase α1 (Ser23) Antibody
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Ser23 of rat Na,K-ATPase α1
- 应用范围：
W
- 宿主：
Rabbit
- 库存：
大量
- 供应商：
CST
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 适应物种：
R
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | R | Endogenous | 100 kDa | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-Na,K-ATPase α1 (Ser23) Antibody recognizes endogenous levels of Na,K-ATPase α1 only when phosphorylated at Ser23. The residue number, Ser23, is based on the sequence of the immature form of the protein, corresponding to Ser18 of the mature cleaved form. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser23 of rat Na,K-ATPase α1. Antibodies are purified using protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from PC-12 cells, untreated or TPA-treated, using Phospho-Na,K-ATPase α1 (Ser23) Antibody (upper) or total Na/K ATPase α1 Antibody #3010 (lower). |
| Background | The Na,K-ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving sodium export and potassium import across the plasma membrane against their electrochemical gradients. It is composed of a catalytic α subunit and a β subunit (reviewed in 1). Several phosphorylation sites have been identified for the α1 subunit. Tyr10 is phosphorylated by an as yet undetermined kinase (2), Ser16 and Ser23 are phosphorylated by PKC, and Ser943 is phosphorylated by PKA (3-5). All of these sites have been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters, altering trafficking and kinetic properties of Na,K-ATPase. Altered phosphorylation in response to angiotensin II stimulates activity in the rat proximal tubule (6). Na,K-ATPase is also involved in other signal transduction pathways. Insulin regulates its localization in differentiated primary human skeletal muscle cells, and this regulation is dependent on ERK1/2 phosphorylation of the α subunit (7). Na,K-ATPase and Src form a signaling receptor complex that affects regulation of Src kinase activity and, subsequently, its downstream effectors (8,9).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验to 1 liter with Qcheck to make sure pH is 3.0(2) Affigel Blue Running Buffer10 mM K2HPO4 2.28 g K2HPO40.15 M NaCl 8.2 g NaCl0.02% azide 0.2 g Na azideup to 1 liter with Q(3) 1.4 M NaCl8.1 g NaClup to 100 ml with Q(4) Saturated NH4SO4767 g NH4SO4add 1 liter Q
1.4 M NaCl 81 g NaCl 40% isopropanol 400 ml isopropanol up to 1 liter with Q check to make sure pH is 3.0 Affigel Blue Running Buffer 10 mM K2HPO4 2.28 g K2HPO4 0.15 M NaCl 8.2 g NaCl 0.02% azide 0.2 g Na azide
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠白介素 -23(IL-23)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 IL-23 单抗包被于酶标板上，标准品和样品中的 IL-23 与单抗结合，加入生物素化的抗大鼠 IL-23 ，形成免疫复合物连接
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