- 询价
- Cell Signaling Technology
- 3059
- USA
- 2025年12月21日
- W
- Rabbit
- H,M,R,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
HSPB8/HSP22 Antibody
- 抗原：
synthetic peptide corresponding to human HSPB8/HSP22
- 应用范围：
W
- 宿主：
Rabbit
- 供应商：
CST
- 级别：
详见MSDS文件
- 库存：
大量
- 保质期：
详见说明书
- 适应物种：
H,M,R,Mk
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 22 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | HSPB8/HSP22 Antibody detects endogenous levels of total HSPB8/HSP22 protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human HSPB8/HSP22. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from various cell lines using HSPB8/HSP22 Antibody. |
| Background | HSPB8 (HSP22) is a member of the small heat shock protein superfamily and the human protein is most closely related to HSP27. Similar to most other small HSPs (sHSPs), HSPB8 is predominantly transcribed in skeletal muscle and heart (1). In a two hybrid screen, HSPB8 interacted preferentially with a triple aspartate form of HSP27 which mimics HSP27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type HSP27 (2). HSPB8 has two binding domains (N- and C-terminal) that are specific for different binding partners, and has the ability to bind to itself and other sHSPs such as HSPB7 and HSPB2 (3). The chaperone-like activity is of great importance to the function of HSP22 in various processes including proliferation, apoptosis and macroautophagy (4). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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