- 询价
- Cell Signaling Technology
- 2425
- USA
- 2026年05月29日
- W
- Rabbit
- H,M,R,Mk
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- 文献和实验
- 技术资料
- 抗体英文名：
eIF4A (F52) Antibody
- 抗原：
synthetic peptide corresponding to residues surrounding Phe52 of human eIF4A
- 应用范围：
W
- 宿主：
Rabbit
- 保质期：
详见说明书
- 供应商：
CST
- 库存：
大量
- 适应物种：
H,M,R,Mk
- 级别：
详见MSDS文件
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 48 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | eIF4A (F52) Antibody detects endogenous levels of total eIF4A1, eIF4A2 and eIF4A3 proteins. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe52 of human eIF4A. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from various cell types using eIF4A (F52) Antibody. |
| Background | A variety of factors contribute to the important biological event of initiation of translation. The eIF4F complex of translation initiation factors binds to the 5' m7 GTP cap to open up the mRNA secondary structure and allow small ribosome subunit binding (1). eIF4A, an eIF4 complex component that acts as an ATP-dependent RNA helicase, unwinds the secondary structure of the 5' mRNA untranslated region to mediate ribosome binding (2,3). In addition, eIF4A has recently been shown to repress Dpp/BMP signalling in a translation-independent manner in Drosophila (4,5). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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Identification of Protein Interactions by Far Western Analysis
far western blotting, a method of identifying protein?protein interactions. In a far western blot, one protein of interest is immobilized on a solid support membrane, then probed with a non?antibody protein. As described, far western blots can be used
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
protein), beta 1, 88kDa (CTNNB1), mRNA 1165 NM_003753 Homo sapiens eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa (EIF3S7), mRNA 1363 NM_004541 Homo sapiens NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa (NDUFA1
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