Reptin/RuvBL2 Antibody

8959:

Western Blotting

Reptin/RuvBL2 Antibody recognizes endogenous levels of total Reptin/RuvBL2 protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly263 of human Reptin/RuvBL2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from various cell lines using Reptin/RuvBL2 Antibody.

Reptin/RuvBL2 and Pontin/RuvBL1 are closely related members of the ATPase Associated with diverse cellular Activities (AAA+) super family of proteins that show homology to bacterial RuvB that drives branch migration of Holliday junctions (1). Both proteins can be found together as part of chromatin remodeling and modification complexes such as INO80, TIP60, SRCAP, and Uri1 that are involved in transcription regulation (1,2). Reptin, as part of these complexes, has been implicated in oncogenic transformation driven by c-Myc, β-catenin, and E1A by acting as an essential transcriptional coregulator of target genes (2-7). Reptin also plays a role in modulating cellular responses to hypoxia. Hypoxia induced methylation of Reptin by the methyltransferase G9a leads to its recruitment to hypoxia responsive promoters where it negatively regulates transcription of these genes (8). In addition to transcriptional regulatory roles, Reptin also participates in the telomerase biogenesis processes as part of the telomerase complex and in DNA damage response as part of the TIP60 acetyltransferase complex that stimulates ATM kinase activity necessary for phosphorylation of proteins involved in both checkpoint activation and DNA repair (9,10).

1. Jha, S. and Dutta, A. (2009) Mol Cell 34, 521-33.
2. Gallant, P. (2007) Trends Cell Biol 17, 187-92.
3. Huber, O. et al. (2008) Cancer Res 68, 6873-6.
4. Kim, J.H. et al. (2005) Nature 434, 921-6.
5. Bauer, A. et al. (2000) EMBO J 19, 6121-30.
6. Wood, M.A. et al. (2000) Mol Cell 5, 321-30.
7. Dugan, K.A. et al. (2002) Oncogene 21, 5835-43.
8. Lee, J.S. et al. (2010) Mol Cell 39, 71-85.
9. Venteicher, A.S. et al. (2008) Cell 132, 945-57.
10. Sun, Y. et al. (2010) Cell Cycle 9, 930-6.

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For Research Use Only. Not For Use In Diagnostic Procedures.