- 询价
- Cell Signaling Technology
- 8566
- USA
- 2025年11月11日
- W, IP
- H,M,R,Hm,Mk
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Ser21 of human GSK-3α protein
- 应用范围：
W, IP
- 级别：
详见MSDS文件
- 供应商：
CST
- 适应物种：
H,M,R,Hm,Mk
- 保质期：
详见说明书
- 库存：
大量
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R Hm Mk | Endogenous | 46 GSK-3beta, 51 GSK-3alpha | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb recognizes endogenous levels of GSK-3α and GSK-3β proteins only when phosphorylated at Ser21 or Ser9, respectively. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser21 of human GSK-3α protein. Western Blotting
Western blot analysis of extracts from COS-7 cells, λ phosphatase- or PDGF-treated (100 μg/ml, 15 min), and PC-3 cells, untreated or λ phosphatase-treated, using Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb (upper) and GSK-3α/β (D75D3) XP® Rabbit mAb #5676 (lower). Western Blotting
Western blot analysis of extracts from GSK-3α (-/-) and GSK-3β (-/-) mouse embryonic fibroblasts (MEF), untreated or insulin-treated (100 ng/ml, 20 min) , using Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb (upper) and GSK-3α/β (D75D3) XP® Rabbit mAb #5676 (lower). (MEF GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada). |
| Background | Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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- 作者
- 内容
- 询问日期
文献和实验. Fig. 5.1. Inhibition of GSK-3 activity. A Depletion of both GSK-3α and GSK-3β is required for a decrease of β-catenin phosphorylationat Ser33/37. Panc04.03 cells were transfected with shControl, shGSK-3α, and shGSK-3β vectors, as describedin
TBS 和 5% NGS 中封闭样品。市售的含有酪蛋白的封闭溶液与磷酸化的一抗结合后易减弱信号；因此，我们建议不使用含有酪蛋白的封闭剂进行磷酸化特异性抗体的检测。以上针对封闭步骤所提建议均是在以 SignalStain. Boost IHC Detection Reagent 作为检测试剂的情况下提出的。如果选择使用其他检测试剂，我们则建议使用与二抗来源相同的血清进行封闭。抗体：Phospho–Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718样本：石蜡
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
3.1 and 3.6 ). This will provide a “specific activity” measurement relative to total protein in the cell, and is themost accurate measure of total GSK3 activity, or2. Immunoblot using antibodies to phospho-Ser21 (GSK3a)or phospho-Ser9 (GSK3b) (see Sections 3.1 –
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