Phospho-NF-κB p105 (Ser933) (1

4806:

Immunoprecipitation , Western Blotting

Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb detects endogenous levels of p105NF-κB only when phosphorylated at serine 933.

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to amino acids around Ser933 of NF-κB p105.

Western Blotting

Western blot analysis of extracts from Vero cells, untreated or treated with TNF-α #2169 (20 ng/ml) for the times indicated, using Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb #4806 (upper) and NF-κB p105/p50 Antibody #3035 (lower).

Western Blotting

Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or treated with TNF-α (#2169, 20 ng/ml) plus Calyculin A (#9902, 50 nM) for 5 minutes, using Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb.

Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).

Following IKK-mediated phosphorylation of p105 NF-κB at multiple sites (Ser921, 923, 927, and 933) on its carboxy-terminus, SCFbeta-TrCP mediated processing produces the 50kDa active form p50 (12,13).

1. Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
2. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
3. Haskill, S. et al. (1991) Cell 65, 1281-9.
4. Thompson, J.E. et al. (1995) Cell 80, 573-82.
5. Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63.
8. Chen, Z.J. et al. (1996) Cell 84, 853-62.
9. Senftleben, U. et al. (2001) Science 293, 1495-9.
10. Coope, H.J. et al. (2002) EMBO J 21, 5375-85.
11. Xiao, G. et al. (2001) Mol Cell 7, 401-9.
12. Heissmeyer, V. et al. (2001) Mol. Cell. Biol. 21, 1024-1035.
13. Orian, A. et al. (2000) EMBO J. 19, 2580-2591.

• Parameswaran, N. et al. (2006) J Biol Chem 281, 34159-70. Applications: Western Blotting
• Minami, M. et al. (2008) J Biol Chem 283, 9692-703. Applications: Western Blotting
• Windheim, M. et al. (2008) Mol Cell Biol 28, 1783-91. Applications: Western Blotting

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• 3035 NF-κB1 p105/p50 Antibody
• 4808 Phospho-NF-κB p105 (Ser933) (178F3) Rabbit mAb (IHC Specific)
• 3039 Phospho-NF-κB p65 (Ser468) Antibody
• 3033 Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb
• 3034 NF-κB p65 Antibody
• 4954 RelB Antibody
• 4727 c-Rel Antibody
• 4882 NF-κB2 p100/p52 Antibody
• 3017 NF-κB2 p100/p52 (18D10) Rabbit mAb (Human Specific)
• 4810 Phospho-NF-κB2 p100 (Ser866/870) Antibody
• 9936 NF-κB Pathway Sampler Kit
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

For Research Use Only. Not For Use In Diagnostic Procedures.