- 询价
- Cell Signaling Technology
- 9569
- USA
- 2025年11月04日
- W, IP
- Rabbit
- M,R,H
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- 详细信息
- 技术资料
- 抗体英文名：
Non-phospho PTEN (Ser380/Thr382/Thr383) Antibody
- 抗原：
synthetic peptide corresponding to residues surrounding Ser380/Thr382/Thr383 of human PTEN
- 应用范围：
W, IP
- 宿主：
Rabbit
- 保质期：
详见说明书
- 供应商：
CST
- 库存：
大量
- 适应物种：
M,R,H
- 级别：
详见MSDS文件
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | M R (H) | Endogenous | 54 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Non-phospho PTEN (Ser380/Thr382/Thr383) Antibody detects endogenous levels of PTEN only when dephosphorylated at Ser380, Thr382 and Thr383. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser380/Thr382/Thr383 of human PTEN. Antibodies are purified by peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, λ phosphatase- or PDGF-treated, using Non-phospho PTEN (Ser380/Thr382/Thr383) Antibody (upper) or PTEN (138G6) Rabbit mAb #9559 (lower). The non-phospho-specificity of the antibody was verified by preincubating the antibody with no peptide, with PTEN (Ser380/Thr382/Thr383) non-phosphopeptide or with PTEN (Ser380/Thr382/Thr383) phosphopeptide prior to incubating the membrane. ELISA-Peptide
Non-phospho PTEN (Ser380/Thr382/Thr383) Antibody specificity was determined by peptide ELISA. The graphs depict the binding of the various dilutions of the antibody (1:250, 1:500, 1:1000, 0) to pre-coated tri-non-phospho PTEN (Ser380/Thr382/Thr383) peptide, tri-phospho PTEN (Ser380/Thr382/Thr383) peptide, phospho PTEN (Ser380) peptide, phospho PTEN (Thr382) peptide, and phospho PTEN (Thr383) peptide. |
| Background | PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10).
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| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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