- 询价
- Cell Signaling Technology
- 4113
- USA
- 2025年11月24日
- W, IP, IHC-P, IF-IC, F
- H,M,R,Mk
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Tyr705 of mouse Stat3
- 应用范围：
W, IP, IHC-P, IF-IC, F
- 保质期：
详见说明书
- 库存：
大量
- 供应商：
CST
- 适应物种：
H,M,R,Mk
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC F | H M R Mk | Endogenous | 79, 86 | Mouse IgG1 |
| Protocols |
* Product-specific protocol. |
|---|---|
| Specificity / Sensitivity | Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb detects endogenous levels of Stat3 only when phosphorylated at Tyr705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr705 of mouse Stat3. Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or treated with IFN-α (100 ng/ml) for 5 minutes, using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb (left) or Stat3 Antibody #9132 (right). IHC-P (paraffin)
Immunohistochemical anlysis of paraffin-embedded human breast carcinoma using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded 786-O cells (SignalSlide® Phospho-Stat1/3/5 IHC Controls #8105), untreated (left) or IFN-α-treated (right), using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb. Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (blue) or IFN-α treated (green), using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb. IF-IC
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or IFN-alpha treated (right), using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red). |
| Background | The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验ddH2O and filter as before. pH=3.0.(11) Regeneration buffer (BioRad): BioRad Affi-gel regeneration buffer.2. Procedure(1) For Ammonium sulfate cutFilter Sup. in 1L Costar Filter using pre-filter.Add 50 mM Tris pH7.8 (50 ml of 1 M stock/Liter): 100
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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