- 询价
- Cell Signaling Technology
- 3198
- USA
- 2025年10月04日
- W, IHC-P, IHC-F
- Rabbit
- H,M,R,Mk
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- 详细信息
- 技术资料
- 抗体英文名：
PKM2 Antibody
- 抗原：
synthetic peptide corresponding to the sequence of human PKM2
- 应用范围：
W, IHC-P, IHC-F
- 宿主：
Rabbit
- 库存：
大量
- 适应物种：
H,M,R,Mk
- 供应商：
CST
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IHC-P IHC-F | H M R Mk | Endogenous | 60 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | PKM2 Antibody detects endogenous levels of total PKM2 protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PKM2. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from various tissue and cell lysates using PKM2 Antibody. Western Blotting
Western blot analysis of extracts from various cell lines using PKM2 Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PKM2 Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PKM2 Antibody in the presence of control peptide (left), PKM1-specific peptide (middle) or PKM2-specific peptide (right). |
| Background | Pyruvate kinase, a glycolytic enzyme, catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues (1). The M2 isoform (PKM2), an alternatively-spliced variant of M1, is expressed during embryonic development (1). Studies found that cancer cells exclusively express PKM2 (1-3). PKM2 is shown to be essential for aerobic glycolysis in tumors (Warburg effect) (1). When the M2 isoform is switched to the M1 isoform, aerobic glycolysis is reduced and oxidative phosphorylation is increased in cancer cells (1). These cells also show decreased tumorigenicity in mouse xenografts (1). Recent studies show that the oncogenic forms of FGFR1 directly phosphorylate Tyr105 of PKM2 and thereby inhibit the formation of active tetrameric PKM2 (4). A PKM2 mutant found in cancer cells, in which Tyr105 is replaced by phenylalanine, leads to reduced cell proliferation in hypoxia and tumor growth in xenografts in nude mice (4). These findings suggest that the phosphorylation at Tyr105 is a critical switch for the metabolism in cancer cells that promotes tumor growth (4). |
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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