- 询价
- Cell Signaling Technology
- 2753
- USA
- 2025年08月21日
- W, IP, IHC-P, IF-IC, F
- Rabbit
- H,Mk,M,R
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Ku80 Antibody
- 抗原：
synthetic peptide corresponding to the carboxy terminus of human Ku80
- 应用范围：
W, IP, IHC-P, IF-IC, F
- 宿主：
Rabbit
- 级别：
详见MSDS文件
- 供应商：
CST
- 适应物种：
H,Mk,M,R
- 保质期：
详见说明书
- 库存：
大量
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P IF-IC F | H Mk (M) (R) | Endogenous | 86 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Ku80 antibody detects endogenous levels of total Ku80 protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human Ku80. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of lysates from HeLa, A673, A549 and COS cells, using Ku80 antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using Ku80 Antibody preincubated with control peptide (left) or antigen-specific peptide (right). IHC-P (paraffin)
Immunohistsochemical analysis of paraffin-embedded human lung carcinoma, showing nuclear localiztion, using Ku80 Antibody. Flow Cytometry
Flow cytometric analysis of HeLa cells, using Ku80 Antibody (blue) compared to a nonspecific negative control antibody (red). IF-F
DAPI staining (left) and immunofluorescent staining (right) of paraformaldehyde-fixed HeLa cells, using Ku80 Antibody. |
| Background | Ku is a heterodimeric protein composed of two subunits (Ku70 and Ku80) originally identified by researchers as autoantigens associated with several autoimmune diseases including scleroderma, polymyositis, and systemic lupus erythematosus (1). Ku is an abundant, ubiquitously expressed nuclear protein that binds to and stabilizes the ends of DNA at telomeres or double-stranded DNA breaks (2-5). The Ku70/Ku80 heterodimer has ATP-dependent DNA helicase activity and functions as the DNA-binding regulatory component of DNA-dependent protein kinase (DNA-PK) (6-8). The assembly of the DNA-PK complex at DNA ends is required for nonhomologous end-joining (NHEJ), one mechanism involved in double-stranded DNA break repair and V(D)J recombination (8). DNA-PK has been shown to phosphorylate many proteins, including p53, serum response factor, c-Jun, c-Fos, c-Myc, Oct-1, Sp-1, and RNA polymerase II (1,8). The combined activities of Ku70/Ku80 and DNA-PK implicate Ku in many cellular functions, including cell cycle regulation, DNA replication and repair, telomere maintenance, recombination, and transcriptional activation.
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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