- 询价
- Cell Signaling Technology
- 4746
- USA
- 2025年11月03日
- W, IHC-P, IF-IC, F
- H
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
TRA-1-60(S) (TRA-1-60(S)) Mouse mAb
- 抗原：
human embryonic carcinoma 2102Ep cl
- 应用范围：
W, IHC-P, IF-IC, F
- 适应物种：
H
- 保质期：
详见说明书
- 供应商：
CST
- 库存：
大量
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC F | H | Endogenous | 200-400 | Mouse IgM |
| Protocols |
* Product-specific protocol. |
|---|---|
| Specificity / Sensitivity | TRA-1-60(S) (TRA-1-60(S)) Mouse mAb detects endogenous levels of the neuraminidase-sensitive TRA-1-60 antigen. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with human embryonic carcinoma 2102Ep cl.2A6 cells. Western Blotting
Western blot analysis of extracts from various cell lines using TRA-1-60 (TRA-1-60) Mouse mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded NTERA (positive, left) and Jurkat (negative, right) cell pellets using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb. Flow Cytometry
Flow cytometric analysis of unpermeabilized Jurkat cells (blue) and unpermeabilized NTERA2 cells (green) using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb. IF-IC
Confocal immunofluorescent analysis of nTERA2 cells using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | TRA-1-60 and TRA-1-81 antibodies detect antigens present on the surface of human stem, teratocarcinoma, and embryonic germ cells (1). TRA-1-60(S) reacts with a neuraminidase sensitive epitope of a proteoglycan (2,3), while TRA-1-81 reacts with a neuraminidase insensitive epitope on the same antigen. Recently this antigen has been proposed to be a form of the protein podocalyxin (4). TRA-1-60 is also detected in the serum of patients with germ cell tumors (5,6).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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