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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
3个月
- 英文名:
Hepatitis C virus NS5A Gene ORF cDNA clone expression plasmid, N-His tag
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1 Unit
基因靶点:HCV-NS5A
反应种属:HCV
应用说明:Stable or Transient mammalian expression
cDNA描述:Full length Clone DNA of Hepatitis C virus (HCV) (serotype 1a, isolate H77) NS5A with N terminal His tag.
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文献和实验RT-PCR for the Assessment of Genetically Heterogenous Populations of the Hepatitis C Virus
within the ISDR (6 ,7 ). The current view is that although the role of the NS5A protein in HCV disease progression is important, pathologically relevant viral genomic sequence evolution may not be strictly confined to the ISDR alone. Sequence variation
Use of the Vaccinia Virus/T7 Expression System for Studying HCV Protein Processing
in length (6 –8 ). Because a cell-culture system supporting efficient HCV replication is lacking, efforts to define potential HCV-encoded polypeptides have utilized expression of HCV cDNA in cell-free translation systems and in cell cultures. The HCV
应用,目前主要以准种HCV基因的复杂性(Complexity)或异质性(Heterogeneity)程度来对其进行量化,主要有以下几种方法:①直接测序法〔15〕。针对特定区段RNA序列进行体外逆转录扩增(RT-PCR)得到相应的cDNA,然后用PCR扩增产物直接测序或克隆后测序,根据异质性序列种类(diversity)来评价该区段准种株多寡。②单链构象多态性分析法(Single-strand conformation polymophism analysis,SSCP)〔l6,l7〕。此法是在完成靶
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