- ￥1770
- Sino Biological
- 北京
- VG40266-NY
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
3个月
- 英文名：
Dengue virus DENV-2 NS4B Gene ORF cDNA clone expression plasmid, N-HA tag
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1 Unit
基因靶点：DENV-NS4B
反应种属：DENV
应用说明：Stable or Transient mammalian expression
cDNA描述：Full length Clone DNA of DENV-2 (strain New Guinea C) NS4B with N terminal HA tag.
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文献和实验cDNA Expression Cloning in Mammalian Cells
protocols for expression cloning in mammalian cells. Either calcium phosphate? or liposome?mediated transfection of mammalian cells, or virus infection and liposome?mediated transfection are used to screen pools derived from a cDNA library. cDNA pools
When trying to identify a clone within a cDNA library, which may contain a coat protein (CP) gene, one useful technique may by immunological screening, using antibodies raised against either purified virus or isolated CP. Antibody screening
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
and thus fusion proteins. For example there might be sequence for a fluorescent protein such as GFP or a peptide tag such as HA upstream of MCS (and downstream of promoter) so that an insert in frame will lead to the generation of a fusion protein, an N-terminal
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