pTagBFP-N-HA-GRM2 plasmid

pTagBFP-N-HA-GRM2 plasmid

PVT96262

pTagBFP-N-HA-GRM2 plasmid Information

Plasmid name: pTagBFP-N-HA-GRM2(mouse)
Alias: NM_001160353.1;4930441L02Rik; Gprc1b; mGluR2; mGluR7
Gene lenght: 2616bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Blue
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pTagBFP-N-HA-GRM2 plasmid Description

pTagBFP-N-HA-GRM2 is a plasmid suitable for protein expression in mammalian cells.

pTagBFP-N-HA-GRM2 plasmid Sequence

1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG 61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT 121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA 181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC 241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA 301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC 361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG 421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG 481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT 541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA 601 CCGGACTCAG ATCTCGAGCT CAAGCTTCGA ATTCgccacc atgTACCCAT ACGACGTACC 661 TGATTATGCA atggaatccc tgcttaggtt cctggcactg ctgctgctgc ggggtgctgt 721 ggccgagggc ccggccaaga aggtgctgac cctggagggg gacctggtgc tgggtgggct 781 gttcccagtg caccagaagg gtggcccagc agaggagtgt ggacctgtta atgagcaccg 841 tggcatacag cgcctagagg ccatgctttt tgcactggac cgcatcaacc gcgaccccca 901 tctgctgcct ggggtgcgtt tgggcgcgca catcctcgac agctgctcca aggatacaca 961 cgccctggag caggccctgg actttgtgcg tgcctcactc agccgtgggg ctgatggctc 1021 acgccacatc tgtcctgacg gctcctatgc tactctcagt gatgctccca cagctatcac 1081 cggtgtcatt ggcggctcct acagtgatgt ctccatccag gtggccaatc tcctgcggct 1141 gttccagatc ccacagatca gctatgcctc caccagcgcc aagctgagcg acaagtcccg 1201 ttatgattac tttgctcgca ccgtgccccc agacttcttc caagccaagg ccatggctga 1261 gattctccgc tttttcaact ggacgtatgt gtctacggtg gcatctgagg gtgactatgg 1321 agagacaggc attgaggcct tcgagctcga ggctcgggca cgcaacatct gcgtggccac 1381 ttcggagaag gtgggccgtg ccatgagccg cgcagccttc gagggtgtgg tgcgagccct 1441 gttgcagaag cccagcgccc gtgtggctgt gctcttcacc cggtcagagg atgcccgtga 1501 gctgcttgca gccacccagc gtctcaacgc cagcttcacc tgggtagcca gcgacggctg 1561 gggggcccta gagagcgtgg tggcaggcag tgaaagggct gccgagggcg ccatcaccat 1621 tgagctggcc tcctacccca tcagtgactt tgcctcctac ttccagaact tggatccctg 1681 gaacaacagc aggaaccctt ggttccgtga gttctgggag gagaggttcc gttgcagctt 1741 ccggcagcga gactgcgccg cccactctct gcgggccgta ccctttgaac aggagtcaaa 1801 gatcatgttt gtggtcaatg ccgtgtatgc catggcccac gctctgcaca acatgcaccg 1861 tgccctctgt cccaacacca cccgcctctg cgatgccatg agacctgtca atgggcgccg 1921 cctctacaag gacttcgtgc tcaatgtcaa gtttgatgcc cccttccgcc cagcagatac 1981 cgacgacgag gtccgctttg accgctttgg agatggtatt ggccgctaca acatcttcac 2041 ctatctgcgg gcaggcaatg ggcgctatcg ctaccagaag gtaggctact gggcagaagg 2101 tctgactctg gatactagca tcatcccatg ggcctcccca tcagctggca ccctccctgc 2161 ctctcgctgt agcgagccct gccttcagaa tgaggtgaag agcgtgcagc ccggcgaggt 2221 ctgctgctgg ctctgcatcc cctgtcagcc ctatgagtac aggctggatg agttcacctg 2281 tgctgactgt ggcctgggct actggcctaa tgccagtctg actggatgct ttgagctgcc 2341 ccaggagtac atccgctggg gcgacgcctg ggcggtggga cctgtcacca tcgcctgcct 2401 aggtgccctg gccaccctct ttgtgttggg tgtcttcgtg cggcataacg ccacacccgt 2461 ggtcaaggcc tcggggcggg agctttgcta cattctgctg ggaggcgtct tcctctgcta 2521 ctgcatgacc ttcatcttca tcgccaagcc ctccacggcc gtctgcacct taaggcgcct 2581 cgggttgggg accgccttct ctgtctgcta ctcagccctc ctcaccaaga ccaatcgcat 2641 cgctcgcatc tttggcgggg cccgggaggg tgcccagcgg ccgcgcttca tcagtcccgc 2701 ctcacaggtg gccatctgcc tggcacttat ctcgggccag ctgctcattg ttgctgcctg 2761 gctggtggtg gaggcacctg gcataggcaa ggagacagcc cctgagcggc gggaagtggt 2821 gaccttgcgc tgtaaccacc gtgacgcaag catgcttggc tcgctggcct acaacgtgct 2881 cctcatcgct ctctgcacgc tctatgcctt caagacccgc aagtgcccgg agaacttcaa 2941 cgaggccaag ttcatcggct ttaccatgta caccacctgt atcatctggc tggctttcct 3001 tcccatcttc tacgtcacct ccagtgatta tcgggtgcag accactacga tgtgcgtgtc 3061 cgtcagcctc agtggctctg tggtgctcgg ctgcctcttt gcacccaagc tgcacattat 3121 cctcttccag ccacagaaga acgtggtgag ccaccgggca cctaccagcc gctttggcag 3181 tgctgccccc agggccagcg ccaaccttgg tcaagggtct ggatcccagc ttgttcctac 3241 tgtttgcaat ggccgtgagg tggtagactc aacaacatcg tcgcttGGTA CCgGCGGCGG 3301 AGGGAGTGGA GGAGGGGGAA GCggaggcgg agggagtcaG GATCCACCGG TCGCCACCAT 3361 GAGCGAGCTG ATTAAGGAGA ACATGCACAT GAAGCTGTAC ATGGAGGGCA CCGTGGACAA 3421 CCATCACTTC AAGTGCACAT CCGAGGGCGA AGGCAAGCCC TACGAGGGCA CCCAGACCAT 3481 GAGAATCAAG GTGGTCGAGG GCGGCCCTCT CCCCTTCGCC TTCGACATCC TGGCTACTAG 3541 CTTCCTCTAC GGCAGCAAGA CCTTCATCAA CCACACCCAG GGCATCCCCG ACTTCTTCAA 3601 GCAGTCCTTC CCTGAGGGCT TCACATGGGA GAGAGTCACC ACATACGAAG ACGGGGGCGT 3661 GCTGACCGCT ACCCAGGACA CCAGCCTCCA GGACGGCTGC CTCATCTACA ACGTCAAGAT 3721 CAGAGGGGTG AACTTCACAT CCAACGGCCC TGTGATGCAG AAGAAAACAC TCGGCTGGGA 3781 GGCCTTCACC GAGACGCTGT ACCCCGCTGA CGGCGGCCTG GAAGGCAGAA ACGACATGGC 3841 CCTGAAGCTC GTGGGCGGGA GCCATCTGAT CGCAAACATC AAGACCACAT ATAGATCCAA 3901 GAAACCCGCT AAGAACCTCA AGATGCCTGG CGTCTACTAT GTGGACTACA GACTGGAAAG 3961 AATCAAGGAG GCCAACAACG AGACCTACGT CGAGCAGCAC GAGGTGGCAG TGGCCAGATA 4021 CTGCGACCTC CCTAGCAAAC TGGGGCACAA GCTTAATTAA AGCGGCCGCG ACTCTAGATC 4081 ATAATCAGCC ATACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT CCCACACCTC 4141 CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG TTAACTTGTT TATTGCAGCT 4201 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA 4261 CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTAAGGCGT AAATTGTAAG 4321 CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA 4381 ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG 4441 TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG 4501 GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT 4561 TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG 4621 AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC 4681 GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC 4741 GCTTAATGCG CCGCTACAGG GCGCGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC 4801 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC 4861 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTCCT GAGGCGGAAA GAACCAGCTG 4921 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 4981 CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 5041 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 5101 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 5161 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG 5221 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAGATC GATCAAGAGA CAGGATGAGG 5281 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA 5341 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT 5401 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT 5461 GAATGAACTG CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG 5521 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT 5581 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC 5641 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC 5701 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA 5761 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG 5821 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT 5881 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG 5941 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC 6001 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA 6061 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG 6121 ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC 6181 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG 6241 GAGTTCTTCG CCCACCCTAG GGGGAGGCTA ACTGAAACAC GGAAGGAGAC AATACCGGAA 6301 GGAACCCGCG CTATGACGGC AATAAAAAGA CAGAATAAAA CGCACGGTGT TGGGTCGTTT 6361 GTTCATAAAC GCGGGGTTCG GTCCCAGGGC TGGCACTCTG TCGATACCCC ACCGAGACCC 6421 CATTGGGGCC AATACGCCCG CGTTTCTTCC TTTTCCCCAC CCCACCCCCC AAGTTCGGGT 6481 GAAGGCCCAG GGCTCGCAGC CAACGTCGGG GCGGCAGGCC CTGCCATAGC CTCAGGTTAC 6541 TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG 6601 ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG 6661 TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC 6721 TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG 6781 CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC 6841 CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC 6901 CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC 6961 GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT 7021 TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT 7081 GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC 7141 GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT 7201 TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA 7261 GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT 7321 TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT 7381 ATTACCGCCA TGCAT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.