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- 文献和实验
- 技术资料
- 规格：
100 µg
蛋白名称：DBT蛋白, DBT protein
蛋白构建：A DNA sequence encoding the human DBT (NP_001909.3) (Gly62-Lys482) was expressed with a polyhistidine tag at the N-terminus.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 95 % as determined by SDS-PAGE.
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：His
蛋白分子量：The recombinant human DBT consists of 441 amino acids and predicts a molecular mass of 48.8 kDa.
蛋白NP号：NP_001909.3
蛋白氨基酸序列：Gly62-Lys482
蛋白标签：N-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验一、简介：1 细胞质膜资料 1895年,Overton从研究细胞透性得出"细胞膜由连续的脂类物质组成"。 1925年 Gorter&Grendel:用脂单分子膜技术测定细胞膜中脂分子的总面积，提出："细胞膜是由双层脂分子组成"。 1935年 Danielli&Davson：从测定膜的表面张力得出细胞膜的"三明治结构模型"，即蛋白质－脂-蛋白质。 1959年 Robertson:用电镜观察生物膜提出
蛋白分离纯化概述（An overview of protein isolation）
Isolating a protein may be compared to playing a game of golf. In golf, the player is faced with a series of problems, each unique and yet similar to problems previously encountered. In facing each problem the player must analyse
In order to carry out detailed studies of a protein (structure,enzyme activity, amino acid sequence, etc) it has to be very pure How is a single protein isolated from all the other proteins in the cell
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