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- 0.23 pg/mL - 15 pg/mL
- 杭州
- EK202HS
- 2025年12月15日
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- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
联科生物
- 检测范围：
0.23 pg/mL - 15 pg/mL
- 检测方法：
双抗夹心法
- 应用：
ELISA
- 适应物种：
Mouse
- 标记物：
HRP
- 样本：
血清，血浆，细胞培养上清及其他生物学样本
- 规格：
96T/48T
| 规格： | 96T | 产品价格： | ￥3400.0 |
|---|---|---|---|
| 规格： | 48T | 产品价格： | ￥2000.0 |
| 背景介绍 | 白细胞介素2(IL-2)是15kDa的糖蛋白，在人类中，由位于4号染色体q26–28区域的单基因编码。它是免疫系统中的一类信号分子，调控对具有免疫功能的白血球(白细胞，通常是淋巴细胞)的活性。IL-2是机体对微生物感染的天然应答，区别外来和自身抗原。IL-2通过与IL-2受体来发挥功能，其受体由3条链组成，分别为α链(CD25)、β链(CD122)和γ链(CD132)。
监测血清中的IL-2含量为多种病理状态提供更详细的视角，包括癌症、传染病、移植排斥、多发性硬化症、类风湿性关节炎、系统性红斑狼疮和I型糖尿病等。 |
| 检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的IL-2与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-2的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。 |
| 组分 | 包被抗IL-2单克隆抗体的96孔聚苯乙烯酶标板 小鼠IL-2冻干标准品 IL-2检测抗体 标准品稀释液 辣根过氧化物酶标记的链霉亲和素 信号增强剂浓缩液 信号增强剂稀释液 检测缓冲液(10×) 底物(TMB) 终止液 洗液(20×) 封板膜 |
| 分析类型 | 夹心ELISA |
| 样本类型 | 血清、血浆，细胞培养上清及其他生物学样本 |
| 板式 | 96孔板，可拆 |
| 保存 | 试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。 |
| 运输条件 | 4℃蓝冰运输 |
| 样本体积 | 20 μl |
| 灵敏度 | 0.05 pg/ml |
| 标曲图 |  |
| 标准曲线范围 | 0.23 - 15 pg/ml |
| 回收率范围 | 82 %-98 % |
| 平均回收率 | 0.92 |
| 板间变异系数 | 4.5 % - 4.9 % |
| 板内变异系数 | 4.4 % - 8.9 % |
IL2 分子靶点信息概述
- 分子名：IL2, interleukin 2
- 基因家族：Interleukins
- 别名：IL-2; TCGF
- 全称：T cell growth factor
IL2 分子靶点综述
白细胞介素2(IL-2)是15kDa的糖蛋白，在人类中，由位于4号染色体q26–28区域的单基因编码。它是免疫系统中的一类信号分子，调控对具有免疫功能的白血球(白细胞，通常是淋巴细胞)的活性。IL-2是机体对微生物感染的天然应答，区别外来和自身抗原。IL-2通过与IL-2受体来发挥功能，其受体由3条链组成，分别为α链(CD25)、β链(CD122)和γ链(CD132)。监测血清中的IL-2含量为多种病理状态提供更详细的视角，包括癌症、传染病、移植排斥、多发性硬化症、类风湿性关节炎、系统性红斑狼疮和I型糖尿病等。
小鼠 Mouse Il2 分子靶点信息
- 分子名：Il2, interleukin 2
- 别称：
- IL-2
- Il-2
- 基因序列：NCBI_Gene: 16183
- 蛋白序列：
- UniprotKB: P04351
- UniprotKB: P70291
- UniprotKB: P70292
- UniprotKB: P70293
- UniprotKB: P70294
- UniprotKB: Q0PGS4
- UniprotKB: Q546X1
- UniprotKB: Q8BHA4
- UniprotKB: Q91Y49
- UniprotKB: Q9QUS8
小鼠 Mouse Il2 靶点分子功能（预测）
Enables cytokine activity and interleukin-2 receptor binding activity. Involved in negative regulation of T-helper 17 cell differentiation. Acts upstream of or within several processes, including extrinsic apoptotic signaling pathway in absence of ligand; positive regulation of macromolecule metabolic process; and regulation of lymphocyte activation. Located in extracellular space. Is expressed in liver; omental bursa; thymus; and thymus primordium. Used to study Sjogren's syndrome and inflammatory bowel disease. Human ortholog(s) of this gene implicated in several diseases, including Takayasu's arteritis; auditory system disease (multiple); autoimmune disease (multiple); carcinoma (multiple); and neurodegenerative disease (multiple). Orthologous to human IL2 (interleukin 2).
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捕获分子的构象。尽管其优异的保护性能，但形成的层很容易溶解，因此稳定过的ELISA板储存后无需额外洗涤，样品可以直接添加入其中。 除了保持分析性能，现代固相稳定剂也有助于优化生产过程。更长的保质期允许更多的生产批次，因为试剂盒可以保存更长的时间。这不仅降低了成本，因为它节省了批量放行和机器设置时间，而且还减少了批间差异，因为客户可以延长使用同一批的时间。现代稳定剂（如Liquid Plate Sealer®）的高批次间一致性进一步补充了这一点。更长的保质期也有利于手工实验室分析，因为它们经常
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Mouse IL-2 High Sensitivity ELISA Kit（小鼠白介素2 高敏ELISA试剂盒）
￥2000 - 3400
