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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken Cellular tumor antigen p53,TP53
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken Cellular tumor antigen p53, TP53 ELISA KIT
ELI-03053c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken Cellular tumor antigen p53, TP53 ELISA KIT is for the quantitative in vitro determination of TP53 concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken Cellular tumor antigen p53, TP53 ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of TP53 in the sample, this Chicken Cellular tumor antigen p53, TP53 ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus TP53 concentration. The concentration of TP53 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验Interaction of p53 with Cellular Proteins
Cellular proteins that interact with p53 play a major role in both positive and negative regulation of this tumor suppressor and can fine-tune its response to specific cellular stresses. As a consequence, p53 biology will not be complete
Stability and Ubiquitination of the Tumor Suppressor Protein p53
its recognition and rapid degradation by the 26S proteasome. The process of ubiquitination requires the concerted action of three classes of cellular enzymes, designated E1, E2, and E3 (reviewed in refs. 1 and 2 ). Ubiquitin is first activated in an ATP
肿瘤抗原(tumor antigen)泛指在肿瘤发生、发展过程中新出现或过度表达的抗原物质。机体产生肿瘤抗原的可能机制为:①基因突变;②细胞癌变过程中使原本不表达的基因被激活;③抗原合成过程的某写环节发生异常(如糖基化异常导致蛋白质特殊降解产物的产生);④胚胎时期抗原或分化抗原的异常、异位表达;⑤某些基因产物尤其是信号转导分子的过度表达;⑥外源性基因(如病毒基因)的表达。 肿瘤
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