- ￥3480
- LSM BIO
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-03050d
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测狗血清，血浆等样本中目标蛋白的浓度
- 适应物种：
狗
- 标记物：
Canine Cellular tumor antigen p53,TP53
- 样本：
狗的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Canine Cellular tumor antigen p53, TP53 ELISA KIT
Product Name:Canine Cellular tumor antigen p53, TP53 ELISA KIT
Packing:96T
Catalog No.:ELI-03050d
Gene Name:Canine Cellular tumor antigen p53, TP53
Detect Range:15.625-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Canine Cellular tumor antigen p53, TP53
Alternative Name:Cellular tumor antigen p53,Cellular tumor antigen p53, TP53
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine Cellular tumor antigen p53, TP53 ELISA KIT allows for the in vitro quantitative determination of Canine Cellular tumor antigen p53, TP53 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: Canine Cellular tumor antigen p53, TP53 ELISA KIT 96 Tests Operating instruction FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING! Synonyms Cellular tumor antigen p53,Tumor suppressor p53,TP53,p53,BCC7; LFS1; TRP53; antigen NY-CO-13; cellular tumor antigen p53; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53; tumor protein p53 Search name Canine Cellular tumor antigen p53 ELISA KIT ,Canine Tumor suppressor p53 ELISA KIT ,Canine TP53 ELISA KIT ,Canine p53 ELISA KIT ,Canine BCC7 ELISA KIT ,Canine LFS1 ELISA KIT ,Canine TRP53 ELISA KIT ,Canine antigen NY-CO-13 ELISA KIT ,Canine cellular tumor antigen p53 ELISA KIT ,Canine p53 tumor suppressor ELISA KIT ,Canine phosphoprotein p53 ELISA KIT ,Canine transformation-related protein 53 ELISA KIT ,Canine tumor protein p53 ELISA KIT Intended use This immunoassay kit allows for the in vitro quantitative determination of Canine Cellular tumor antigen p53 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids. Test principle The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cellular tumor antigen p53. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cellular tumor antigen p53 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cellular tumor antigen p53, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cellular tumor antigen p53 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
The microtiter plate provided in
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
"(本产品仅供体外科研用途，不可用于临床诊断!)
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文献和实验Interaction of p53 with Cellular Proteins
Cellular proteins that interact with p53 play a major role in both positive and negative regulation of this tumor suppressor and can fine-tune its response to specific cellular stresses. As a consequence, p53 biology will not be complete
Stability and Ubiquitination of the Tumor Suppressor Protein p53
its recognition and rapid degradation by the 26S proteasome. The process of ubiquitination requires the concerted action of three classes of cellular enzymes, designated E1, E2, and E3 (reviewed in refs. 1 and 2 ). Ubiquitin is first activated in an ATP
肿瘤抗原（tumor antigen）泛指在肿瘤发生、发展过程中新出现或过度表达的抗原物质。机体产生肿瘤抗原的可能机制为：①基因突变；②细胞癌变过程中使原本不表达的基因被激活；③抗原合成过程的某写环节发生异常（如糖基化异常导致蛋白质特殊降解产物的产生）；④胚胎时期抗原或分化抗原的异常、异位表达；⑤某些基因产物尤其是信号转导分子的过度表达；⑥外源性基因（如病毒基因）的表达。 肿瘤
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