- ￥2000 - 3400
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- 0.25 pg/mL - 16 pg/mL
- 杭州
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- 2025年12月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
联科生物
- 检测范围：
0.25 pg/mL - 16 pg/mL
- 检测方法：
双抗夹心法
- 应用：
ELISA
- 适应物种：
Human
- 标记物：
HRP
- 样本：
血清，血浆，细胞培养上清及其他生物学样本
- 规格：
96T/48T
| 规格： | 96T | 产品价格： | ￥3400.0 |
|---|---|---|---|
| 规格： | 48T | 产品价格： | ￥2000.0 |
| 背景介绍 | 白细胞介素4(IL-4)是约18-20kDa的单体细胞因子，可诱导幼稚辅助性T细胞(Th0细胞)分化为Th2细胞。Th2细胞受IL-4活化后，随后正反馈分泌IL-4。分泌IL-4诱导Th0细胞分化的细胞类型尚未得到鉴定，但近期研究表明，嗜碱性粒细胞可能是该效应细胞。IL-4与IL-13密切相关，且具有相似的功能。
IL-4还可促使横纹肌肉瘤发生有丝分裂、去分化和转移。在过敏性哮喘病人中可观察到IL-4和其它Th2细胞因子参与了气道炎症。 |
| 检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-4抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的IL-4与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-4的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570-630 nm)测定吸光度值。 |
| 组分 | 包被抗IL-4单克隆抗体的96孔聚苯乙烯酶标板 人IL-4冻干标准品 IL-4检测抗体 标准品稀释液 辣根过氧化物酶标记的链霉亲和素 信号增强剂浓缩液 信号增强剂稀释液 检测缓冲液(10×) 底物(TMB) 终止液 洗液(20×) 封板膜 |
| 分析类型 | 夹心ELISA |
| 样本类型 | 血清、血浆，细胞培养上清及其他生物学样本 |
| 板式 | 96孔板，可拆 |
| 保存 | 试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。 |
| 运输条件 | 4℃蓝冰运输 |
| 样本体积 | 血清血浆：20 μl 细胞培养上清：100μl |
| 灵敏度 | 0.03 pg/ml |
| 标曲图 |  |
| 标准曲线范围 | 0.25-16 pg/ml |
| 回收率范围 | 94 %-105 % |
| 平均回收率 | 0.98 |
| 板间变异系数 | 3.6 %-8.1 % |
| 板内变异系数 | 4.2 %-4.8 % |
IL4 分子靶点信息概述
- 分子名：IL4, interleukin 4
- 基因家族：Interleukins
- 别名：BSF1; IL-4; BCGF1; BCGF-1; MGC79402
- 全称：B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; B cell growth factor 1
IL4 分子靶点综述
IL-4是II型辅助T细胞(Th2细胞)分泌的细胞因子。IL-4是一种多效性细胞因子，在调节T、B淋巴细胞和其他类型细胞的增殖、分化、凋亡，促进以Th2细胞为特征的免疫应答过程中发挥重要作用。IL-4多重生物效应依赖于细胞类型和分化状态，研究表明其在介导过敏性疾病、自身免疫性疾病、感染性疾病、肿瘤等疾病的免疫反应中有多重作用，对肿瘤、自身免疫性疾病和感染性疾病等有治疗作用，并且IL-4对疫苗免疫应答具有调节作用。
人 Human IL4 分子靶点信息
- 分子名：IL4, interleukin 4
- 别称：
- B cell growth factor 1
- B_cell stimulatory factor 1
- B-cell stimulatory factor 1
- BCGF-1
- BCGF1
- binetrakin
- BSF-1
- BSF1
- IL-4
- interleukin 4 variant 2
- interleukin-4
- lymphocyte stimulatory factor 1
- MGC79402
- pitrakinra
- 基因序列：NCBI_Gene: 3565
- 蛋白序列：UniProtKB: P05112
人 Human IL4靶点分子功能（预测）
Predicted to enable cytokine activity. Involved in several processes, including negative regulation of cellular response to transforming growth factor beta stimulus; negative regulation of complement-dependent cytotoxicity; and positive regulation of macromolecule metabolic process. Acts upstream of or within positive regulation of cell migration and positive regulation of cell population proliferation. Predicted to be located in external side of plasma membrane. Predicted to be active in extracellular space. Implicated in several diseases, including asthma (multiple); autoimmune disease (multiple); hepatitis B; hepatitis C; and pancreatic cancer (multiple). Biomarker of several diseases, including allergic rhinitis; alopecia universalis; anogenital venereal wart; autoimmune disease (multiple); and liver disease (multiple).
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文献和实验该产品被引用文献
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- IF = 7.561 Identification of Robust Biomarkers for Early Predicting Efficacy of Subcutaneous Immunotherapy in Children With House Dust Mite-Induced Allergic Rhinitis by Multiple Cytokine Profiling. Frontiers in Immunology (2022)
- IF = 7.561 ILC2 Cells Promote Th2 Cell Differentiation in AECOPD Through Activated Notch-GATA3 Signaling Pathway. Frontiers in Immunology (2021)
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Quantification of Human IgG and Related Fc Fusion Proteins by a Human IgG/Fc Capture ELISA
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R&D Systems Quantikine® 人高分子量脂联素ELISA试剂盒
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时对一个样本中的75个目的蛋白进行精确定量。2、样本需要量小。仅需要50μL溶液就可进行一次检测。3、更高的灵敏度。分析灵敏度高达2.8pg/mL。4、更高的灵敏度、更宽的检测范围和更好的重复性。CBA技术使蛋白定量的分析灵敏度高达pg/ml级别,检测范围达0-5000pg/ml，所有分析只需一组标准曲线。根据抗体的荧光强度对目的蛋白定量，排除了ELISA反应中酶联放大产生的假阳性。5、检测对象的灵活组合—BD CBA Flex Set。CBA kit是同时检测试剂盒配好的6个指标，形式比较固定
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文献支持
Human IL-4 High Sensitivity ELISA Kit（人白介素4 高敏ELISA试剂盒）
￥2000 - 3400
